FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22727
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA440319.373432481189774TruSeq Adapter, Index 2 (96% over 27bp)
CAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT19858.734104809257712TruSeq Adapter, Index 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA11134.897258767105205TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA10834.765257183086197RNA PCR Primer, Index 32 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3381.4872178466141595RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3381.4872178466141595RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA3001.3200158401900823TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA2140.9416112993355921TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC2120.9328111937343249TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1640.7216086593039116TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA1230.5412064944779338TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG1220.5368064416773001TruSeq Adapter, Index 2 (96% over 27bp)
CAGTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1130.49720596647159765RNA PCR Primer, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA1080.4752057024684296TruSeq Adapter, Index 2 (96% over 27bp)
GCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA1060.46640559686716243RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA970.42680512166145995Illumina PCR Primer Index 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA950.4180050160601927TruSeq Adapter, Index 2 (96% over 27bp)
AGCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA860.37840454085449027RNA PCR Primer, Index 32 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC790.347604171250055TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA760.3344040128481542RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA700.30800369604435257TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC680.2992035904430853RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT660.2904034848418181TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA620.2728032736392837TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG600.2640031680380165TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA590.25960311523738283RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA540.2376028512342148Illumina PCR Primer Index 2 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA540.2376028512342148TruSeq Adapter, Index 2 (96% over 27bp)
CAGCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA530.23320279843358122TruSeq Adapter, Index 2 (96% over 27bp)
NTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA510.22440269283231398RNA PCR Primer, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA490.21560258723104675TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA480.21120253443041317RNA PCR Primer, Index 32 (96% over 28bp)
CAGATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA480.21120253443041317RNA PCR Primer, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA460.20240242882914594RNA PCR Primer, Index 32 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA420.1848022176266115RNA PCR Primer, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG410.1804021648259779RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA400.17600211202534433TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA390.1716020592247107TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA370.16280195362344346TruSeq Adapter, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA350.15400184802217629RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA350.15400184802217629RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC340.14960179522154265TruSeq Adapter, Index 2 (96% over 27bp)
GTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAA340.14960179522154265TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA330.14520174242090905Illumina Single End Adapter 1 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA320.14080168962027545TruSeq Adapter, Index 2 (96% over 27bp)
CAGTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA320.14080168962027545RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC300.13200158401900824TruSeq Adapter, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA300.13200158401900824RNA PCR Primer, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA290.12760153121837461RNA PCR Primer, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAA290.12760153121837461RNA PCR Primer, Index 2 (96% over 27bp)
GACAGACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA280.12320147841774101RNA PCR Primer, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAG270.1188014256171074RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA270.1188014256171074TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATT260.1144013728164738TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGG260.1144013728164738TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAA260.1144013728164738TruSeq Adapter, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAA250.11000132001584019RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAG250.11000132001584019RNA PCR Primer, Index 2 (96% over 27bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA230.10120121441457297RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAACAA230.10120121441457297RNA PCR Primer, Index 2 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)