FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448088
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138263.0855546231990143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82841.848743996714931No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50901.1359375836889183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50441.1256717430504721No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44350.9897609398153934No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33220.7413722304547321No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30140.6726357322668761No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17270.3854153648390495No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17030.3800592740711646TruSeq Adapter, Index 2 (96% over 27bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12310.27472282230276196No Hit
CAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT7560.1687168591883737TruSeq Adapter, Index 2 (96% over 25bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.14974737105211475No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.13769616682437377No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6100.1361339736837407No Hit
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5220.11649497420149614TruSeq Adapter, Index 2 (96% over 27bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.10243523593579833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)