FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14879
Sequences flagged as poor quality0
Sequence length75
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA291319.5779286242355TruSeq Adapter, Index 2 (96% over 27bp)
CAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT11837.950803145372673TruSeq Adapter, Index 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6764.543316083070099TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4352.92358357416493RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2071.3912225283957256TruSeq Adapter, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1811.2164796021237985RNA PCR Primer, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1721.1559916661065932RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1651.1089454936487668TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC1380.9274816855971504TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1130.759459641104913TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA830.5578331877142282TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG720.4839034881376437TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA690.4637408427985752Illumina PCR Primer Index 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA650.4368573156798172TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA600.4032529067813697TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA570.38309026144230124TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA430.2889979165266483TruSeq Adapter, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT420.2822770347469588TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC420.2822770347469588TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG370.24867262584851135TruSeq Adapter, Index 2 (96% over 27bp)
GCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA370.24867262584851135RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA330.22178909872975333TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA310.20834733517037435TruSeq Adapter, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA310.20834733517037435TruSeq Adapter, Index 2 (96% over 27bp)
CAGTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA280.18818468983130587RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA270.18146380805161638TruSeq Adapter, Index 2 (96% over 27bp)
NTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA270.18146380805161638RNA PCR Primer, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA260.1747429262719269RNA PCR Primer, Index 32 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA260.1747429262719269RNA PCR Primer, Index 32 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA250.16802204449223737Illumina PCR Primer Index 2 (96% over 25bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA240.16130116271254788Illumina PCR Primer Index 2 (96% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC240.16130116271254788RNA PCR Primer, Index 32 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA240.16130116271254788TruSeq Adapter, Index 2 (96% over 27bp)
CAGATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA240.16130116271254788RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC220.1478593991531689TruSeq Adapter, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACAAA210.1411385173734794RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAACA210.1411385173734794RNA PCR Primer, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA200.1344176355937899RNA PCR Primer, Index 2 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA200.1344176355937899TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA200.1344176355937899RNA PCR Primer, Index 32 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAAAA200.1344176355937899RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA190.12769675381410042Illumina Single End Adapter 1 (96% over 25bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAGAA190.12769675381410042RNA PCR Primer, Index 2 (96% over 27bp)
NAGCTTAATCGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCT190.12769675381410042TruSeq Adapter, Index 2 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATT180.12097587203441093TruSeq Adapter, Index 2 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAC180.12097587203441093RNA PCR Primer, Index 2 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACG170.11425499025472141TruSeq Adapter, Index 2 (96% over 27bp)
AGCTTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA170.11425499025472141RNA PCR Primer, Index 32 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAAAAA170.11425499025472141TruSeq Adapter, Index 2 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAATA160.10753410847503192RNA PCR Primer, Index 32 (96% over 28bp)
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTG160.10753410847503192TruSeq Adapter, Index 2 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGA160.10753410847503192RNA PCR Primer, Index 2 (96% over 27bp)
CACATCTCCGAGCCCACGAGACGCTCCTCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAA150.10081322669534243RNA PCR Primer, Index 32 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)