FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14879
Sequences flagged as poor quality0
Sequence length75
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATTAAGCTGCTGTCTCTTATACACATCTGACGCTGCCGACGAGTTAGTTCGTGTAGATCTCGGTGGTCGCCGT11767.903756972914847Illumina RNA PCR Primer (100% over 24bp)
CTTATACACATCTGACGCTGCCGACGAGTTAGTTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA770.5175078970360911Illumina RNA PCR Primer (100% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.1478593991531689No Hit
ATACACATCTGACGCTGCCGACGAGTTAGTTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAA210.1411385173734794Illumina RNA PCR Primer (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)