FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328786
Sequences flagged as poor quality0
Sequence length75
%GC31

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76692.3325202411294885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46051.4006070818100527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36431.1080155481072795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.6852481553350812No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTTAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA20590.6262432098690334RNA PCR Primer, Index 44 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.3430803014726904No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8690.26430565778348225No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.2585268229182508No Hit
TTATACACATCTCCGAGCCCACGAGACGAGTTAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7490.22780775337149392RNA PCR Primer, Index 24 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGAGTTAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA7310.22233306770969566RNA PCR Primer, Index 44 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7240.220204023285663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3300.10036923713296796No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)