FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451702
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62021.3730291209691345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37450.8290864330908431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.5519125441109404No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA23750.52578912645948Illumina PCR Primer Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17580.3891946460276908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.16448897724606046No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7080.15674050590876284No Hit
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6890.15253419289708703Illumina PCR Primer Index 3 (96% over 27bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.1403580236527622No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5360.11866230390832895RNA PCR Primer, Index 25 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4920.10892136851286911No Hit

[WARN]Adapter Content

Adapter graph