FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244402
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT11220.45907971293197275No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCGGGTGCTGGAAGG8610.3522884428114336No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA8500.3477876613121006No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG7370.3015523604553154No Hit
CTTGTGCGTCGCCCGGCGAAAGGGAAACCGGTTAACATTCCGGTACCTGGATGTGGATTCTCCACGGCAACGTAA7230.2958240930925279No Hit
GTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCC6970.28518588227592245No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAG6790.2778209670951956No Hit
GTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCC6670.27291102364137776No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAACCGGTTAACA6650.27209269973240807No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT6180.25286208787162134No Hit
ACGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCA6090.24917963028125795No Hit
GTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGG5820.23813225751016767No Hit
TGCCAAGGGCGTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTT5740.2348589618742891No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA5580.2283123706025319No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT5390.22053829346732023No Hit
CCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTA5230.21399170219556304No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC5100.20867259678726036No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA5070.20744511092380585No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC4920.2013076816065335No Hit
CTCCACGGCAACGTAACTGAAAGCGAAGACGACGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACGGCC4830.19762522401617008No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA4790.19598857619823076No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT4790.19598857619823076No Hit
GGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC4730.19353360447132184No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCGGG4590.1878053371085343No Hit
GAGTACGACCTGGCGTGAAAACTATTCCTTCCAGCGAATTTTCAAGGGACGTCGGGGGCGCACCGGATCCCACAA4560.1865778512450798No Hit
GTGCACCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT4530.18535036538162536No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC4480.18330455560920125No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG4320.17675796433744406No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGCCG4280.17512131651950474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4280.17512131651950474No Hit
GGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAG4270.17471215456501993No Hit
CATCAGGATCGGTCGATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTTCGCGCAGGGGTTTGACACC4150.16980221111120203No Hit
GGTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAA4130.1689838872022324No Hit
ATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTTCGCGCAGGGGTTTGACACCCGAACACTCGCATAG4120.16857472524774755No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGC4090.1673472393842931No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACC4090.1673472393842931No Hit
GCCGAAGCCTGCCAAGGGCGTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTC4010.1640739437484145No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG3970.1624372959304752Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA3960.16202813397599036No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3940.16120981006702073Clontech Universal Primer Mix Long (96% over 26bp)
GTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTC3900.15957316224908144No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3890.1591640002945966No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC3860.15793651443114215No Hit
GTGTGTAATACGGCCAGCCGGGACTGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGACCCG3850.1575273524766573No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT3830.15670902856768767No Hit
GCCTTGGGGGTGACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAAC3790.15507238074974838No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3730.1526174090228394No Hit
CCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAGCGTCGAATCTACTGTAGCGGCGAGGTAT3720.1522082470683546No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGTATTCCGGCACCTTAACCTCGCGTTCGGTTCAT3670.15016243729593048No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3600.14729830361453672No Hit
GTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT3600.14729830361453672No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT3590.14688914166005188No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT3580.14647997970556706No Hit
ACCCATACCTCGCCGCTACAGTAGATTCGACGCTGTAGCGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTTG3520.1440250079786581No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAA3480.14238836016071882No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT3440.14075171234277953No Hit
GCCGTGGAGAATCCACATCCAGGTACCGGAATGTTAACCGGTTTCCCTTTCGCCGGGCGACGCACAAGTGCGCAC3410.13952422647932503No Hit
ACCCCGGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGA3390.1387059025703554No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC3320.13584176888896163No Hit
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC3300.13502344497999197No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT3230.1321593112985982No Hit
ATTACACACTGGGCTATAACACCTCCACCGAAGTGGAGGCCACATTCCCAGAGCCTTTATCCGGCCGCCGAAACT3190.1305226634806589No Hit
GCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA3160.12929517761720444No Hit
ACCCGGGGTCACCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAGCGTCGAATCTACTGTAG3160.12929517761720444No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT3150.12888601566271962No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGAAAACGTTACTGCTATCC3150.12888601566271962No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA3130.12806769175374996No Hit
GTTATAGCCCAGTGTGTAATACGGCCAGCCGGGACTGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTG3130.12806769175374996No Hit
GTCATAGAGGGTGAGAATCCCGTATGTGACTGGAAATGTTAACCTATGTAAAGCTCCTTCGACGAGTCGAGTTGT3080.12602188198132586No Hit
CCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCT3010.12315774829993209No Hit
CCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCTTCCCAGGGCCCCCGC3000.12274858634544726No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2990.12233942439096243No Hit
CTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGAACTCT2960.12111193852750796No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG2870.11742948093714455No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2870.11742948093714455No Hit
TGTTTAAACTGTTCGGCCGGTCTTCTGACCGGTTTACTCAGTTTGGACAGGCCAGCATCAGTTTCGGCGGCCGGA2840.11620199507369007No Hit
GTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAA2830.11579283311920524No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCG2830.11579283311920524No Hit
GGGCTATAACACCTCCACCGAAGTGGAGGCCACATTCCCAGAGCCTTTATCCGGCCGCCGAAACTGATGCTGGCC2810.11497450921023558No Hit
GCACTAGAGGCCGTTCTACCCGGGGTCACCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAG2770.1133378613922963No Hit
GTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGG2760.11292869943781148No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTATTCAGGCATAGTTCACCATCTTTCG2670.10924624184744805No Hit
GTGTAATACGGCCAGCCGGGACTGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGACCCGTC2650.1084279179384784No Hit
GGTTAGTCGATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAAC2600.10638210816605428No Hit
TCCCAGGGCCCCCGCCGTCGTCTTCGCTTTCAGTTACGTTGCCGTGGAGAATCCACATCCAGGTACCGGAATGTT2590.10597294621156945No Hit
GAGCTAGGGTTTAATGGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATT2570.10515462230259981No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG2550.10433629839363018No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGG2550.10433629839363018No Hit
GGGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATACTCAAATGAGAACTTTGAGGACTGAAGTGG2490.10188132666672123No Hit
TGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTGACGTGCAAATCGATCGTCAA2470.10106300275775158No Hit
GGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTAAGAAGAAAAGAGA2460.10065384080326675No Hit
CCACAAAGGTGTGGGACTCTACCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACGGGCCGTTA2460.10065384080326675No Hit
ACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACT2450.10024467884878192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)