FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244402
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTACTTGTGCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT8800.3600625199466453No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG7300.29868822677392165No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCGGGTGCTGGAAGG7000.2864133681393769No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGA6230.2549078976440455No Hit
CTTGTGCGTCGCCCGGCGAAAGGGAAACCGGTTAACATTCCGGTACCTGGATGTGGATTCTCCACGGCAACGTAA6030.24672465855434897No Hit
GTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCC5840.23895058141913733No Hit
GTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCC5800.237313933601198No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAAGCACGTGAAATTGTTGAAAGGGAAG5450.22299326519422918No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTT4990.20417181528792727No Hit
CCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTA4840.1980343859706549No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCACTTGTGCGTCGCCCGGCGAAAGGGAAACCGGTTAACA4800.19639773815271563No Hit
GTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGG4780.19557941424374597No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4670.1910786327444129No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAAAAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC4580.18739617515404947No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA4520.18494120342714054No Hit
TGCCAAGGGCGTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTCCCAGTGCTT4360.17839461215538335No Hit
GTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGCCG4090.1673472393842931No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3880.15875483834011178No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA3830.15670902856768767No Hit
GGCCGGTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAG3790.15507238074974838No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3750.15343573293180907No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG3720.1522082470683546No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG3680.1505715992504153No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACA3650.14934411338696083No Hit
CTCCACGGCAACGTAACTGAAAGCGAAGACGACGGCGGGGGCCCTGGGAAGAGTTCTCTTTTCTTCTTAACGGCC3650.14934411338696083No Hit
GGCTAGTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC3570.14607081775108222No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAAT3450.14116087429726434No Hit
CATCAGGATCGGTCGATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTTCGCGCAGGGGTTTGACACC3320.13584176888896163No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACAACCGGG3310.1354326069344768No Hit
GTGTGTAATACGGCCAGCCGGGACTGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGACCCG3220.1317501493441134No Hit
GCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCACACGGAACCTTTCCCCACTTCAGTCCTCAAAGTTCTCA3200.13093182543514373No Hit
GTAGAGTCCCACACCTTTGTGGGATCCGGTGCGCCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTC3200.13093182543514373No Hit
ACGCACAAGTGCGCACTTTAAAACGGAGTTTCCCTATGGCTTAGGATCGACTAACCCGTGTCCAACTGCTGTTCA3180.13011350152617407No Hit
GTGCACCATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGT3170.12970433957168928No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTTTTGTGGTGTCTGAT3110.1272493678447803No Hit
GCCGAAGCCTGCCAAGGGCGTCCACAACCGGGTGCTGGAAGGCGCCGGCCCGTGAAGGCTAGTGCCGACCTGCTC3100.12684020589029552No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTATGCTCTTACTCAAATCCATCCGAAGACATCAGGAT3040.12438523416338654No Hit
AAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGT3040.12438523416338654No Hit
ATGGTGCACCTTGCGGTTCCCACCTCCGTTCACTTTCATTTCGCGCAGGGGTTTGACACCCGAACACTCGCATAG3020.1235669102544169No Hit
GTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTGAAAACTATTCC3000.12274858634544726No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2990.12233942439096243Clontech Universal Primer Mix Long (96% over 26bp)
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT2990.12233942439096243No Hit
CCCCCAAGGCTTCGTCACTGACCTCCACGCCTGCCTACTCGCTACAGCGTCGAATCTACTGTAGCGGCGAGGTAT2960.12111193852750796No Hit
CATCAGACACCACAAAAGGTGTTAGTTCATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAATCCGCTAAGGA2940.12029361461853832No Hit
GTGCCGACCTGCTCCCAGTGCTTCCACCCAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGT2940.12029361461853832No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAGTTGAACGTGGACATTTGAATGTAT2870.11742948093714455No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACC2870.11742948093714455No Hit
GTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGC2850.1166111570281749No Hit
ACCCATACCTCGCCGCTACAGTAGATTCGACGCTGTAGCGAGTAGGCAGGCGTGGAGGTCAGTGACGAAGCCTTG2760.11292869943781148No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGGTTGTTAAGCGTAC2750.11251953748332665No Hit
GTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAGACAGTCA2750.11251953748332665No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2740.11211037552884182Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAATGATTAGAGGCCTTGGGGTTGAAACAACCTTAACC2730.111701213574357No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTA2700.11047372771090254No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTTC2660.10883707989296322No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGTTTTCAGTTTTATGAGGTAAAGCGAAT2590.10597294621156945No Hit
ATTACACACTGGGCTATAACACCTCCACCGAAGTGGAGGCCACATTCCCAGAGCCTTTATCCGGCCGCCGAAACT2590.10597294621156945No Hit
GTTATAGCCCAGTGTGTAATACGGCCAGCCGGGACTGAGGTCCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTG2540.10392713643914535No Hit
CCCCCGACGTCCCTTGAAAATTCGCTGGAAGGAATAGTTTTCACGCCAGGTCGTACTCATAACCGCAGCAGGTCT2460.10065384080326675No Hit
GTCTAGATGAACTAACACCTTTTGTGGTGTCTGATGAGCGTGTATTCCGGCACCTTAACCTCGCGTTCGGTTCAT2460.10065384080326675No Hit
CTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGATCGATTTGCACGTCAGAACCGCTGCG2450.10024467884878192No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)