FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303151
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60031.9802012858278546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG44511.4682451979376614Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT24210.7986119128751018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21010.6930539566090826Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC18490.6099270660495925Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16160.5330676791433971Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA14850.4898548907969955Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14540.47962896378372494Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG12500.4123357666641377Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10290.3394348031179181Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC9930.32755953303799096Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC9650.3183232118647143Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.30743754762478104No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9310.30710767901144975Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9190.30314925565147405Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG8400.27708963519830054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7120.2348664526918928Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6280.20715748917206275Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG5970.19693156215879215Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5660.18670563514552155No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC5540.18274721178554582No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT5340.1761498395189196Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4870.16064601469234804Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA4820.15899667162569148No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4770.15734732855903494Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT4620.1523992993590653No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4580.15107982490574004Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA4480.14778113877242693No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC4330.1428331095724573Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT4190.13821494898581893Clontech Universal Primer Mix Long (96% over 25bp)
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG4190.13821494898581893No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA4140.13656560591916242No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA4110.13557600007916848No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3900.12864875919921095Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3900.12864875919921095Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA3870.12765915335921704No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG3870.12765915335921704No Hit
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG3870.12765915335921704No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA3850.12699941613255442No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3850.12699941613255442Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA3770.12436046722590392Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3680.12139164970592213Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG3550.1171033577326151No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT3490.11512414605262725No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3390.11182545991931414Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA3330.10984624823932629Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG3330.10984624823932629No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC3330.10984624823932629No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA3320.10951637962599496No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT3300.10885664239933235Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3250.1072072993326758Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10588782487935056No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT3180.10489821903935663Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTAC3150.1039086131993627Clontech Universal Primer Mix Long (96% over 25bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC3100.10225927013270614No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3050.10060992706604958No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC3040.10028005845271828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)