FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401569
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCATGGACCTTGAAAATCTATGAAAGGGATACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC12610.3140182633619627No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG12490.31102998488429134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTCAGAGCCAATCCT9990.24877418326613854No Hit
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTA9950.24777809044024815No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9720.24205055669137807Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATGAA7590.18900861371271188No Hit
TTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTA7400.18427717278973227No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGTGGCTC7150.178051592627917No Hit
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATT7080.17630843018260872No Hit
CTCATAGAGAAGCCGTTGGGAGGCATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGCACCATGGAAGTCT7050.17556136056319088No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT6550.16311020023956033No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT6160.1533982951871285No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT5660.1409471348634979Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA5100.1270018353010317No Hit
AGCCACCACAGTCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATT5090.1267528120945591No Hit
TGGTATAACAGATTGGTAGAGCATGACATTAAATTGTTGTGTCGGTGCCCTCTCCATGGACCTTGAAAATCTATG5030.12525867285572342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4820.12002918551979858No Hit
GTATATTAAGTCTGTAATGGATGGCATTCACGTGTCGTCTATGAAAGGCCATCAATTAACAGTCAATTCAGAACT4800.11953113910685335No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT4620.11504872139034637No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTT4400.1095702108479489No Hit
ATCCATTACAGACTTAATATACATCGCACCGCGAAGCACAATGCATATTGTAAAGCCCGAGCCACCACAGTCTTC4300.10707997878322281No Hit
GTTCTGAATTGACTGTTAATTGATGGCCTTTCATAGACGACACGTGAATGCCATCCATTACAGACTTAATATACA4300.10707997878322281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4020.10010732900198968Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)