FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287732
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG50801.7655318143272214Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG41671.4482226516341594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22700.7889285863233844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC15660.5442564608733127Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15130.525836542338009Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14080.48934425090014316No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13760.4782227906524127Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12780.44416331864373787Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC11250.3909888368342764Clontech Universal Primer Mix Long (96% over 26bp)
ATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGTCCATAGCAGGTCCGCCGCAGCTTGCGCC10680.3711787357680063No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10230.35553918229463527Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10150.3527588172327027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTA10010.34789317837432054No Hit
ACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCT9940.3454603589451295No Hit
GTCCTAAGAGATAGGGGAATTCCGTTTCAAAGTGTGCGCTTGTTCGCACGGCCTATCGAAAGGGAATACGGTTAA9850.3423324482504553No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9550.3319060792682079Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9270.3221748015514437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTATCGAAAGGGAATACGGTTAAAATTCCGTAACCGAGATGTAGATTCTGAACGGCAACGTAAATGAACTTGG8880.30862052187452216No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8800.30584015681258947No Hit
CTACAGAATAGCAGTGCTCTACCAACCATTAGACCTTATCTCCGGATAATCCGATTCCAAGGTTATAAGTTGTAA8730.3034073373833985No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC7040.24467212545007158Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6870.23876384969346476Clontech Universal Primer Mix Long (96% over 26bp)
GTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGTCCATAGCAGG6310.21930129425993633No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.21096019907413843No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6070.21096019907413843Clontech Universal Primer Mix Long (96% over 25bp)
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCATAGAATAATGTAGATAAGGGAAGTC5720.19879610192818317No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5440.18906482421141896Clontech SMART CDS Primer II A (96% over 26bp)
GTCCGTACCAGTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGT5350.18593691351674477No Hit
GGGTAGGGTATATCGGGGTCTTGGATAAAGATGAGGGACTATGGCGGGACTACTGTGGCGCAAGCTGCGGCGGAC5280.1835040940875537No Hit
TCGATAGGCCGTGCGAACAAGCGCACACTTTGAAACGGAATTCCCCTATCTCTTAGGACCGACTCACCCATGTCC4960.17238263383982316No Hit
CTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGA4890.16994981441063212No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4850.1685596318796658Clontech Universal Primer Mix Long (96% over 26bp)
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTT4790.1664743580832163No Hit
GGTCCATAGCAGGTCCGCCGCAGCTTGCGCCACAGTAGTCCCGCCATAGTCCCTCATCTTTATCCAAGACCCCGA4600.15987099106112632No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT4530.15743817163193527No Hit
GACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCG4500.15639553473371054No Hit
GGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGTTGAGGACGCACCGGACACTACAG4470.1553528978354858No Hit
GATATACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATT4460.15500535220274422No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4390.15257253277355318Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4230.1470118026496879No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGAC4200.14596916575146315No Hit
GTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTACCCATATCCGCATCAGGTC4200.14596916575146315No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4180.14527407448598Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4050.14075598126033947Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGT3970.13797561619840684No Hit
GTAGAGCACTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTT3970.13797561619840684No Hit
CCATTAGACCTTATCTCCGGATAATCCGATTCCAAGGTTATAAGTTGTAAAGGAGAAAAGATAACTCTTCCCAGG3840.13345752297276633No Hit
CTCTTAGGACCGACTCACCCATGTCCAACTGCTGTTACCATGGAACCCTTCCCCACTTCAGTCTTCAAGGTTCTC3830.13310997734002475No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3780.13137224917631685Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3760.1306771579108337Clontech Universal Primer Mix Long (96% over 26bp)
GCTAAGGAGTGTGTAACAACTCAACGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA3750.13032961227809212No Hit
GTCTAATGGTTGGTAGAGCACTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAA3730.12963452101260894No Hit
GAATAGCAGTGCTCTACCAACCATTAGACCTTATCTCCGGATAATCCGATTCCAAGGTTATAAGTTGTAAAGGAG3680.12789679284890107No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3460.12025078892858633Clontech Universal Primer Mix Long (96% over 26bp)
GTTGAGGACGCACCGGACACTACAGAATAGCAGTGCTCTACCAACCATTAGACCTTATCTCCGGATAATCCGATT3440.11955569766310317No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTTAATTAG3410.11851306076487843No Hit
GTGTGTAACAACTCAACGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGC3400.11816551513213684No Hit
CTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCTATGGACTA3400.11816551513213684No Hit
GCTATAGATTGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAGTATTCTCAGGCGCTTGAGAGAACTCTGG3360.11677533260117054No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCGACTTTCGTCTTTGCAGAGTGCTGTGTTTTTATTAAACAG3340.11608024133568737No Hit
GTCTTACACTCGATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCATAGAATAATGTAG3310.11503760443746264No Hit
ATCCGGAGTAGAACGGCCTCTAGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAGTGAGAACCTTGAAGACTGA3280.1139949675392379No Hit
GTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTACCCATATCCG3270.11364742190649632No Hit
TCTTTATCCAAGACCCCGATATACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGA3210.11156214811004685No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG3200.11121460247730527Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGGGCCCTCACCGGCGTCTCCAAGTTCATTTACGTTGCCGTTCAGAATCTACATCTCGGTTACGGAATTTT3190.11086705684456369No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA3160.10982441994633896Clontech Universal Primer Mix Long (96% over 26bp)
GAATAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCATAGGGTAGGGTA3140.10912932868085579No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATTGTAT3140.10912932868085579No Hit
ACTTATAACCTTGGAATCGGATTATCCGGAGATAAGGTCTAATGGTTGGTAGAGCACTGCTATTCTGTAGTGTCC3140.10912932868085579No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT3050.1060014179861816Clontech Universal Primer Mix Long (96% over 25bp)
GCCCTGGGAAGAGTTATCTTTTCTCCTTTACAACTTATAACCTTGGAATCGGATTATCCGGAGATAAGGTCTAAT3040.10565387235344001No Hit
ATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTTTTTCA2960.10287350729150738No Hit
TGGGTGAGTCGGTCCTAAGAGATAGGGGAATTCCGTTTCAAAGTGTGCGCTTGTTCGCACGGCCTATCGAAAGGG2880.10009314222957474No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA2880.10009314222957474Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)