FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287732
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG36211.2584627361572576Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG29931.040204078795546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16950.5890898474969763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11580.4024578427147484Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11550.40141520581652373Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10400.3614474580512421Clontech Universal Primer Mix Long (96% over 26bp)
ATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTA9650.3353815355956237No Hit
ATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGTCCATAGCAGGTCCGCCGCAGCTTGCGCC8880.30862052187452216No Hit
ACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTATTCT8660.3009745179542074No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG8420.2926334227684095Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7990.2776889605605216Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7610.26448222651634157No Hit
CTACAGAATAGCAGTGCTCTACCAACCATTAGACCTTATCTCCGGATAATCCGATTCCAAGGTTATAAGTTGTAA7140.24814758177748739No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC7130.2478000361447458Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC7040.24467212545007158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCTAAGAGATAGGGGAATTCCGTTTCAAAGTGTGCGCTTGTTCGCACGGCCTATCGAAAGGGAATACGGTTAA6980.24258685165362212No Hit
GCCTATCGAAAGGGAATACGGTTAAAATTCCGTAACCGAGATGTAGATTCTGAACGGCAACGTAAATGAACTTGG6940.2411966691226558No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG6620.23007520887492527Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG6610.22972766324218372Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCGTACCCATATCCGCATCAGGTCCCCAAGGTGATCAGCCTCTAGTCCATAGAATAATGTAGATAAGGGAAGTC5190.18037618339287947No Hit
GTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGTCCATAGCAGG5100.17724827269820528No Hit
GGGTAGGGTATATCGGGGTCTTGGATAAAGATGAGGGACTATGGCGGGACTACTGTGGCGCAAGCTGCGGCGGAC4980.17307772510530633No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4910.17064490567611526Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4700.16334644738854212Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGACTCGGGT4480.15570044346822737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4450.15465780657000264No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4440.15431026093726108Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGA4310.14979216771162052No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4140.1438838919550137Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGCTATGTTTT3950.1372805249329237No Hit
CCCCTTGTCCGTACCAGTTCATAGTTGGTAGTTAATTGTATACCGGACGACCGAAATCTGGTCAAGGGCGCCGAC3880.13484770550373262No Hit
GATATACCCTACCCTATGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATT3790.13171979480905843No Hit
GGTCCATAGCAGGTCCGCCGCAGCTTGCGCCACAGTAGTCCCGCCATAGTCCCTCATCTTTATCCAAGACCCCGA3760.1306771579108337No Hit
ACATTATTCTATGGACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTT3730.12963452101260894No Hit
GTAGAGCACTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTT3640.12650661031793475No Hit
CCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGT3630.12615906468519317No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3630.12615906468519317Clontech Universal Primer Mix Long (96% over 26bp)
GACTAGAGGCTGATCACCTTGGGGACCTGATGCGGATATGGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCG3580.12442133652148528No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.1174704238666537No Hit
GTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTACCCATATCCG3360.11677533260117054No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG3330.11573269570294581Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTACGATCGAGTGTAAGACTTTTTCATTCCCTCGGATTTTCAAGGGCCGTTGAGGACGCACCGGACACTACAG3310.11503760443746264No Hit
GTCTAATGGTTGGTAGAGCACTGCTATTCTGTAGTGTCCGGTGCGTCCTCAACGGCCCTTGAAAATCCGAGGGAA3250.11295233064101318No Hit
GTCCTCAACGGCCCTTGAAAATCCGAGGGAATGAAAAAGTCTTACACTCGATCGTACCCATATCCGCATCAGGTC3130.10878178304811423No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC3130.10878178304811423Clontech Universal Primer Mix Long (96% over 26bp)
GAATAGCAGTGCTCTACCAACCATTAGACCTTATCTCCGGATAATCCGATTCCAAGGTTATAAGTTGTAAAGGAG3100.10773914614988948No Hit
TCGATAGGCCGTGCGAACAAGCGCACACTTTGAAACGGAATTCCCCTATCTCTTAGGACCGACTCACCCATGTCC3090.1073916005171479No Hit
GTCCATAGCAGGTCCGCCGCAGCTTGCGCCACAGTAGTCCCGCCATAGTCCCTCATCTTTATCCAAGACCCCGAT3050.1060014179861816No Hit
GCTAAGGAGTGTGTAACAACTCAACGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA3000.10426368982247369No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG2950.1025259616587658Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGTAACAACTCAACGGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCATACCTCGC2920.10148332476054105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)