FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755060
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG150471.99282176250894Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG120721.598813339337271Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT57090.7560988530712791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC49850.6602124334489974Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG43500.5761131565703388Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG41630.5513469128281197Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36600.4847296903557333No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT36020.4770481816014621Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAGCCAGTCCAGTCAGGTTGCCCCAACCAGTCCGGCCAAGTCCAGATCAGTTCCGTCAATGGCCCGACGTA34280.45400365533864856No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC32370.42870765237199693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA32000.42380737954599634Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30500.4039414086297778Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTTGACAGTCCTTGAAAATCCGACGGAACGTATAAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTC25260.33454295022912084No Hit
GGCTAGGACCGGAGATGGGATGTCTTTGGCAGCTCTCGGGCGTCTGGCGTACGATTAACAACCGACTTAGAACTG24440.32368288612825474No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21700.28739437925462874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21330.28249410642862816Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21100.2794479908881414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20070.2658066908590046Clontech Universal Primer Mix Long (96% over 26bp)
GTCCAGATCAGTTCCGTCAATGGCCCGACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA19240.2548141869520303No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18780.2487219558710566No Hit
CCTCTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACGTATAAGTCTCACGCCTGGTCGTA18320.24262972479008293No Hit
GTCAAGGACGCACCGGACACAACAGAGGTGTTGTGCTCTACCCGTCATCTAACCCTAGCTCCGGATAATCCGATT17610.2332264985564061No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGA17000.22514767038381056No Hit
GTCTACGACCCTGAAATCGGATTATCCGGAGCTAGGGTTAGATGACGGGTAGAGCACAACACCTCTGTTGTGTCC16800.22249887426164808No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATACGTTCCGTCGG16330.21627420337456627No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCT16330.21627420337456627Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC15820.20951977326305193No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG15760.20872513442640317Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC15600.20660609752867323Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT15540.2058114586920245No Hit
GTATAAGTCTCACGCCTGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACA15300.20263290334542952No Hit
GATCTGGACTTGGCCGGACTGGTTGGGGCAACCTGACTGGACTGGCTAGGACCGGAGATGGGATGTCTTTGGCAG15180.201043625672132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT14970.1982623897438614Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTCAACACCACTTTCTGGCCATCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGT14930.19773263051942894No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTCG14920.19760019071332077No Hit
GTTCTAAGTCGGTTGTTAATCGTACGCCAGACGCCCGAGAGCTGCCAAAGACATCCCATCTCCGGTCCTAGCCAG14760.1954811538155908No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC14540.1925674780812121Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTAGACGGTTAAGGAGAAAAGAGAACTCTTCCCGGGGCCCTTGCCAGCGTCTCCAACTTCATTTACGTTGCC14250.1887267237040765No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14050.18607792758191402No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG12870.17045003046115542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTC12760.16899319259396606No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT12610.1670065955023442Clontech SMART CDS Primer II A (96% over 26bp)
ACCCATACCTCACCGTCAGCGTTGAAGTGACGCGCTGACGAGTAGGCAGGCGTGGGGGTTTGTGAAGAAGCCTTG12550.16621195666569544No Hit
CGATTATCCGTGTCGCCTATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGATGTGGATCTTTGACGGCAAC12540.16607951685958733No Hit
GCCCCAACCAGTCCGGCCAAGTCCAGATCAGTTCCGTCAATGGCCCGACGTACCCAACCCTTAGAGCCAATCCTT12500.1655497576351548No Hit
GACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATT12200.1615765634519111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.15720604985034303No Hit
GGTTGGGGCAACCTGACTGGACTGGCTAGGACCGGAGATGGGATGTCTTTGGCAGCTCTCGGGCGTCTGGCGTAC11650.15429237411596428No Hit
CAACAGAGGTGTTGTGCTCTACCCGTCATCTAACCCTAGCTCCGGATAATCCGATTTCAGGGTCGTAGACGGTTA11630.15402749450374806No Hit
CCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAATCGTACGCCAGACGCCCGAGAGCTGCCAAAGACATCCCAT11350.1503191799327206No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11320.1499218605143962Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11180.14806770322888246Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG10880.14409450904563875Clontech Universal Primer Mix Long (96% over 26bp)
GTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACGTATAAGTCTCACGCCTGGTCGTACTCAT10730.1421079119540169No Hit
GAGTACGACCAGGCGTGAGACTTATACGTTCCGTCGGATTTTCAAGGACTGTCAAGGACGCACCGGACACAACAG10400.1377373983524488No Hit
CTCCTTAACCGTCTACGACCCTGAAATCGGATTATCCGGAGCTAGGGTTAGATGACGGGTAGAGCACAACACCTC10390.1376049585463407No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCCA10020.1327046857203401No Hit
TCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTCGGTTGTTAATCGTAC9790.12965857017985324No Hit
GACTTATACGTTCCGTCGGATTTTCAAGGACTGTCAAGGACGCACCGGACACAACAGAGGTGTTGTGCTCTACCC9750.12912881095542075No Hit
GCCAAAGACATCCCATCTCCGGTCCTAGCCAGTCCAGTCAGGTTGCCCCAACCAGTCCGGCCAAGTCCAGATCAG9700.12846661192488015No Hit
GGTATGGGTAACACGCTTGAGCGCCATCCATTTTCAGGGCTAGTTCATTCGGCAGGTGAGTTGTTACACACTCCT9640.12767197308823142No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC9630.12753953328212328Clontech Universal Primer Mix Long (96% over 26bp)
ATCACATTGTGTCAACACCACTTTCTGGCCATCACAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCC9620.12740709347601514No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATACGTTCCGTCGGATTTTCAAGGACTGT9570.12674489444547454No Hit
GAGCACAACACCTCTGTTGTGTCCGGTGCGTCCTTGACAGTCCTTGAAAATCCGACGGAACGTATAAGTCTCACG9370.12409609832331205No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG9330.12356633909887955Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA9320.12343389929277145Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTTATACG9250.12250682065001457No Hit
GTCCGTACCAGTTCTAAGTCGGTTGTTAATCGTACGCCAGACGCCCGAGAGCTGCCAAAGACATCCCATCTCCGG9050.11985802452785209No Hit
GCACTAGGGGCTGTTTCACCCAGCATCACTGCCAAGGCTTCTTCACAAACCCCCACGCCTGCCTACTCGTCAGCG9010.11932826530341958No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT9010.11932826530341958Clontech Universal Primer Mix Long (96% over 26bp)
GTACACGATTATCCGTGTCGCCTATCGAAAGGGAATCCGGTTAAGATTCCGGAACCAGGATGTGGATCTTTGACG8750.11588483034460836No Hit
GGGTACGTCGGGCCATTGACGGAACTGATCTGGACTTGGCCGGACTGGTTGGGGCAACCTGACTGGACTGGCTAG8740.11575239053850025No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA8690.11509019150795963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCCAAGGTGAACAGCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8550.11323603422244591No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8520.11283871480412154Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAAGGGTTGGGTACGTCGGGCCATTGACGGAACTGATCTGGACTTGGCCGGACTGGTTGGGGCAACCTGAC8500.11257383519190528No Hit
ACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGCGTGAGACTT8440.11177919635525653No Hit
GATCTGCACTAGGGGCTGTTTCACCCAGCATCACTGCCAAGGCTTCTTCACAAACCCCCACGCCTGCCTACTCGT8390.11111699732471593No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT8110.10740868275368844No Hit
CTCTAAGGGTTGGGTACGTCGGGCCATTGACGGAACTGATCTGGACTTGGCCGGACTGGTTGGGGCAACCTGACT8100.10727624294758033No Hit
CATCTAGACAGCAGGACGGTGGCCATGGAAGTCGGAACCCGCTAAGGAGTGTGTAACAACTCACCTGCCGAATGA7880.10436256721320158No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC7880.10436256721320158Clontech Universal Primer Mix Long (96% over 26bp)
ACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCT7810.10343548857044474No Hit
CCCTTATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCAGGC7690.10184621089714724No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT7670.101581331284931Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)