FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002148479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842527
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC47040.5583203861716004No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA45660.541941089128301No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG38060.4517362648318689No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT29820.353935244805211No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA28480.33803070999505064No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT26140.3102571193564123No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC24690.29304698840511934No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG22010.26123791878479863No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC20360.24165397666781005No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC18410.21850931780227814No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA17620.2091327636977806No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA17520.2079458581149328No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT16580.1967889456361636No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC15900.1887179876727986No Hit
GTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCG15560.18468250869111613No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC14800.17566202626147293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.17494988291176425No Hit
GGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAA14450.17150785672150567No Hit
TTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATT14100.1673536871815384No Hit
GCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGT14030.16652285327354494No Hit
GATAAGAGTTTTGCTGGAGCTCTTTACTTCTGTCGAAACGATTTAGCCTTGATTGCCTGGAATTTTAGGCATTTG13570.16106308759244511No Hit
GGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTT12970.15394165409535837No Hit
GTCTAGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTCGCGGGTGTTGACACAATGTGATTTC12810.1520426051628019No Hit
CAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGC12730.1510930806965237No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA12330.14634545836513252No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT12170.14444640943257606No Hit
GGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCT11910.1413604549171718No Hit
GATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGAT11880.14100438324231745No Hit
ATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGT11580.1374436664937741No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG11460.13601937979435674No Hit
GTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAAC11420.13554461756121763No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA11380.13506985532807853No Hit
GGTTAAGAGTCTCTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAG11340.1345950930949394No Hit
CCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTAA11260.13364556862866117No Hit
CTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGC10610.1259306823401505No Hit
AAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACC10410.12355687117445494No Hit
AGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGC10370.12308210894131583No Hit
CTAGTCGATAGATCAATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGA10130.12023353554248112No Hit
TCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTA10120.12011484498419636No Hit
TACCTACATTGATCTATCGACTAGAGACTCTTAACCTTGGAGACCTGCTGCGGATTCGGTACAGACTGTTGAGAA9720.11536722265280519No Hit
GGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAA8880.10539721575688375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.10254864235804904No Hit
GATAGAAACTAACCTGGCTCACGCCGGTCTAAACTCAAATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAAC8440.10017483119235347No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)