FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42849
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC3080.7188032392821303No Hit
CCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCA2140.4994282246960256No Hit
CTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCA2070.4830917874396135No Hit
ATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCCTT1650.3850731639011412No Hit
GTCTAGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTCGCGGGTGTTGACACAATGTGATTTC1420.331396298630073No Hit
GGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGG1320.30805853112091297No Hit
CCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCG1310.30572475436999696No Hit
TTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATT1200.2800532101099209No Hit
GTGTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCT1090.2543816658498448No Hit
GTTATACACTTTGGTTGGGCTGGCAACGGCCCTTCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAAC1080.2520478890989288No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTA960.2240425680879367No Hit
GTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTC910.21237368433335668No Hit
GGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAA910.21237368433335668No Hit
CTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGTTATACACTTTGGTTGGGCTGGCAACGGCCC830.1937034703260286No Hit
GCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAGCCTGT810.1890359168241966No Hit
GGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATT700.1633643725641205No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGA690.1610305958132045No Hit
GTACATGGGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTT680.15869681906228852No Hit
AAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACC640.14936171205862447No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTT630.14702793530770847No Hit
GCCCAGGACATCTAAGGGCATCACGGACCTGTTATTGCTCAATCTCATTTTGCTAAACACAAATTGTCCTATTAA610.14236038180587646No Hit
GTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAATTAGACAGTCGG590.13769282830404442No Hit
GTATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTC570.1330252748022124No Hit
GTACATGGGAATTCCAGGTTCATGTGAACAGTTTCAGTCCACAATCCCAACTAAGAAGACGTTTTAGTGATTTCC570.1330252748022124No Hit
CTTATACACATCTGACGCTGCCGACGACTCCGGACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA570.1330252748022124Illumina RNA PCR Primer (96% over 33bp)
CCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTAA560.13069149805129643No Hit
TCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAA560.13069149805129643No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTCGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGATTGAGATAACTC540.1260239445494644No Hit
CTCACACCCTTTAGGAAGGGCCGTTGCCAGCCCAACCAAAGTGTATAACAGGCTTAGCAAAACCGCAAAACTTGC540.1260239445494644No Hit
TCCTAAAGGGTGTGAGTTATCAATTAACAATCAATTCAGAACTGGCACGGTCTAGGGAATCCGACTGTCTAATTA540.1260239445494644No Hit
GGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTT480.11202128404396836No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGTGTCAACACCCGCGAGGGCCATCACAATGCTTTGTTTTAA470.10968750729305234No Hit
ATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCGAATTTGCCGACT470.10968750729305234No Hit
GTATCAACGCAGAGTACATGGGGGATAAGGATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTG460.10735373054213634No Hit
GGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAGAGCCAA450.10501995379122034No Hit
GTGTAGTTTCACCTGATAGTTTGTCTACCTCCTTGTGGGGTGACTTTATCTGCTTAATAGGACAATTTGTGTTTA440.10268617704030432No Hit
ATAACAGGCTTAGCAAAACCGCAAAACTTGCGAATCGCAGTTATATAGCCCAGCCCGAGTTATCTCAATCTTCAG430.10035240028938833No Hit
GATTGGCTCTGAAGATTGAGATAACTCGGGCTGGGCTATATAACTGCGATTCGCAAGTTTTGCGGTTTTGCTAAG430.10035240028938833No Hit
GGTCTAGGGAATCCGACTGTCTAATTAAAACAAAGCATTGTGATGGCCCTCGCGGGTGTTGACACAATGTGATTT430.10035240028938833No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)