FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191563
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51222.6737939998851554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32401.6913495821218083No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA19351.0101115559894134Illumina PCR Primer Index 3 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17750.9265881198352499No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15630.8159195669309836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.5136691323481049No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.37115726941006355No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6180.32260927214545604No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.284501704400119No Hit
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5200.271451167501031Illumina PCR Primer Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3290.17174506559199845RNA PCR Primer, Index 25 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.13207143341877084No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.12424111127931803No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2060.10753642404848535No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.10701440257252183No Hit
ACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2000.10440429519270422TruSeq Adapter, Index 25 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)