FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205611
Sequences flagged as poor quality0
Sequence length75
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA121985.932561973824358Illumina PCR Primer Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA48082.3383962920271775RNA PCR Primer, Index 25 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA41522.019347213913653Illumina PCR Primer Index 3 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA15730.7650368900496569TruSeq Adapter, Index 25 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA14040.682842844011264TruSeq Adapter, Index 25 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.6755475144812291No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA8850.43042444227205745Illumina PCR Primer Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA8840.42993808697005514Illumina PCR Primer Index 3 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.3652528318037459No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.3331533818715925No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC6080.2957040236174135Illumina PCR Primer Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG5690.2767361668393228Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA5040.24512307220917168Illumina PCR Primer Index 3 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA4560.2217780177130601Illumina PCR Primer Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA4540.22080530710905547RNA PCR Primer, Index 25 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA4440.21594175408903218Illumina PCR Primer Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1848150147608834No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3610.17557426402283924RNA PCR Primer, Index 41 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA3430.1668198685867974RNA PCR Primer, Index 25 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC3330.16195631556677414Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA3250.15806547315075556Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA3010.14639294590269974Illumina PCR Primer Index 3 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG2940.1429884587886835Illumina PCR Primer Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA2660.1293705103326184RNA PCR Primer, Index 25 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA2640.12839779972861376Illumina PCR Primer Index 3 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC2500.12158882550058119RNA PCR Primer, Index 25 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG2460.11964340429257189RNA PCR Primer, Index 25 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA2430.11818433838656492RNA PCR Primer, Index 25 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA2310.11234807476253703Illumina PCR Primer Index 3 (96% over 27bp)
ACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA2240.10894358764852075TruSeq Adapter, Index 25 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA2090.10164825811848587Illumina PCR Primer Index 3 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)