FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512307
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129062.5191925935035044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79531.552389485211016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61851.2072839137470306No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA58081.133695225714269Illumina PCR Primer Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.7109018615790923No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA21150.41283839572756176RNA PCR Primer, Index 25 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA17730.34608154875884967Illumina PCR Primer Index 3 (96% over 27bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11950.23325857347254675No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10150.1981233908574351No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.14405424872195774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT7000.13663682128098972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT6760.1319521302656415No Hit
TATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA6680.13039056659385875TruSeq Adapter, Index 25 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.11516532079397705Clontech SMART CDS Primer II A (100% over 21bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.10618632968122631No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.10169683412485091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)