FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578548
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118782.0530707910147474No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69191.1959249707889408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57490.9936945594833965No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA35390.6117037825729239Illumina PCR Primer Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.5712577003118151No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA14000.24198510754509564RNA PCR Primer, Index 25 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11360.19635363012230617No Hit
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA10230.17682197501330918Illumina PCR Primer Index 3 (96% over 27bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9670.16714257071150537No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG9070.15677178038814413No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.1545247758180825No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG8280.14311690646238515No Hit
GTGATAGACCGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAAGTCTCCGCTCTCAGTTGCGTTGCC7450.12877064651506875No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCCGGAAGAGCGCTGCACTTTTGCGGCGTTT6420.1109674564599653No Hit
TATCTACATTGTTCTATCAACTAGAGGCTTTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGTAA5820.10059666613660406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)