FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences578548
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64811.1202182014284034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.6583723390280495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27390.4734265782614407No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.28606096641938095No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG8760.15141353872107413No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTCAATGGCCGGAAGAGCGCTGCACTTTTGCGGCGTTT7540.13032626506357295No Hit
GTTCTAAGTTGGTTGTTAAGCGTACGCCGGACGGCCGAAGCCTGCCAAGGGTGCCAGCATCCCAGCGCTGAAAGG7220.12479517689111361No Hit
GTGATAGACCGTTAAGAAGAAAAGAGAACTCTTCTTGGGGCTCCCGCCAAAGTCTCCGCTCTCAGTTGCGTTGCC6690.11563431210547787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.10059666613660406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)