FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582280
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80681.385587689771244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.8401456344026929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38830.6668613038400769No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA26150.44909665453046643Illumina PCR Primer Index 3 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.37782510132582264No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.1284605344507797No Hit
TTATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA7400.12708662499141307RNA PCR Primer, Index 25 (96% over 28bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6560.11266057566806348No Hit
ATACACATCTCCGAGCCCACGAGACTCTGTGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6360.1092258020196469Illumina PCR Primer Index 3 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)