FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250074
Sequences flagged as poor quality0
Sequence length75
%GC23

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71212.847557123091565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42771.7102937530490976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32261.2900181546262306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22140.885337939969769No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA19320.7725713188896086TruSeq Adapter, Index 12 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.36629157769300286No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8480.3390996265105529No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7320.29271335684637345No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.22473347889024847No Hit
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4950.1979414093428345TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3840.15355454785383527TruSeq Adapter, Index 12 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3730.14915584986843894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3210.1283620048465654No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2760.1103673312699441No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTT2640.10556875164951175No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)