FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158890
Sequences flagged as poor quality0
Sequence length75
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA94195.928000503492982TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA41242.5955063251305934TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA35532.2361382088237147TruSeq Adapter, Index 12 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA14530.9144691295865063RNA PCR Primer, Index 12 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA13470.8477563093964379RNA PCR Primer, Index 12 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.6438416514569828No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA6020.378878469381333TruSeq Adapter, Index 12 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.3574800176222544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4980.31342438164768077No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA4910.3090188180502234TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC4670.29391402857322674TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG4040.2542639561961105TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA3550.223425011013909TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA3400.21398451759078607TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA3330.2095789539933287TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA3070.19321543205991565TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC2950.18566303732141734TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA2780.17496381144187803TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA2760.17370507898546164TruSeq Adapter, Index 12 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2620.16489395179054692No Hit
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA2580.16237648687771414TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC2360.14853042985713386TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2330.1466423311725093RNA PCR Primer, Index 48 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG2280.1434955000314683TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG2040.12839071055447165TruSeq Adapter, Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA1870.11769148467493235TruSeq Adapter, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA1830.11517401976209955TruSeq Adapter, Index 12 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAGA1730.10888035748001762RNA PCR Primer, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAA1700.10699225879539304TruSeq Adapter, Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA1650.10384542765435206TruSeq Adapter, Index 12 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACA1610.10132796274151928RNA PCR Primer, Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACTCATTGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA1600.1006985965133111TruSeq Adapter, Index 12 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)