FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002148553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158890
Sequences flagged as poor quality0
Sequence length75
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16251.0227201208383159No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9870.6211844672414878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7170.45125558562527535No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5870.3694379759582101No Hit
CTTATACACATCTGACGCTGCCGACGAAGCCGGCAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA3030.1906979671470829Illumina Single End PCR Primer 1 (100% over 30bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2070.13027880923909624No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1620.10195732896972748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)