FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278019
Sequences flagged as poor quality0
Sequence length75
%GC23

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60212.1656793240749734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37541.3502674277657283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.925836004014114No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22000.7913128239436873RNA PCR Primer, Index 46 (96% over 30bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.6438408885723638No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7380.2654494836683824No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6800.24458760012804878No Hit
ATACACATCTCCGAGCCCACGAGACTATCCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5780.2078994601088415RNA PCR Primer, Index 46 (96% over 30bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.20718008481434722No Hit
TTATACACATCTCCGAGCCCACGAGACTATCCTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4660.16761444361716285Illumina PCR Primer Index 8 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4660.16761444361716285No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3900.14027818242638093No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3340.12013567418054161No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT2840.1021512918181851No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)