FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458998
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127902.7865045163595483No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG23560.5132919969150193No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18280.39825881594255313No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12470.27167874369822964No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA8830.19237556590660523No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8750.19063263892217394No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.1845323944766644No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8190.1784321500311548No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7070.15403117224911655No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6990.15228824526468523No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT6640.1446629397077983No Hit
CAAGGTACACAGAGTAAAATGTTTTTCTTTTTTCAGGACCTTGAACTGAA6580.1433557444694748No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6480.1411770857389357No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6080.13246245081677915No Hit
CCCCGGAGAGGGACTCACGGGTAGCACAACGGCCAGTTCGGCAATAGCAG6020.13115525557845567No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6010.13093738970540175No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5860.12766940160959306No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA5200.1132902539880348No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.10784360716168699No Hit
CTCCAGGAAAGAGCTGATGAGGCTACAACCCAGAAGCAGTCTGGGGAAGA4650.10130763097006958No Hit
ACCCATGACAGTGCAGGTCCCATAGGAATATTCTGTTGAATAACAGGACC4640.10108976509701567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)