FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148925

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148925
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308835
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCAC9160.2965985072935386No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG8870.28720837987922354Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCTAAACCGTGCCAGTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCA7450.24122913529878412No Hit
GTTGTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGG6910.2237440704583354No Hit
CGTATGCACCATGGAAGTCTTTCATAGAGAGATATGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTG6860.2221250829731086No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG6670.21597293052924701Clontech Universal Primer Mix Long (96% over 26bp)
GTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAA6510.21079217057652144No Hit
CTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTATGCACCATGGAAGTCT5860.18974533326857382No Hit
TACCTACATTGTTCTATCGACTAGAGACTCTTAATCTTGGAGACCTGCTGCGGATTCGGTACAGGCTATTGAGAA5840.18909773827448315No Hit
ATGTAGGTAAGGGAAGTCGGCAAATTAGATCCGTAACTTCGGGATAAGGATTGGCTCTGAAGACTGTGGCAGCTC5450.17646963588971457No Hit
GTATAACAGCTGTAATTTCTTAGATCATATTCCCAGTACTTGGATAACTGTGGAAAATCTAGAGCTAATACATGC5170.16740330597244485No Hit
AGCCTGTACCGAATCCGCAGCAGGTCTCCAAGATTAAGAGTCTCTAGTCGATAGAACAATGTAGGTAAGGGAAGT5140.16643191348130879No Hit
GTTCTAAGTTGGTTGTTAAACGTGCGCCGGACGGCCGAAGCCTGCCAAGGGTGTCAGCACCCCAGCGCTGAAAGG4960.16060355853449254No Hit
GTCGGTACCCTCTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCG4880.15801317855812974No Hit
TGGTATAACAGATTGGTAGAGCATGGCATTAAATTGCTGTGTCGGTACCCTCTCCTTGGACCTTGAAAATCTATG4830.156394191072903No Hit
CTTCAGAGCCAATCCTTATCCCGAAGTTACGGATCTAATTTGCCGACTTCCCTTACCTACATTGTTCTATCGACT4750.15380381109654023No Hit
GTGTCAACATCCACCGAGAACCATCACAATGCTTTGTTTTAATTAGACAGTCGGATTCCCTAAACCGTGCCAGTT4490.1453850761733612No Hit
GGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAGAGTTTTC4460.14441368368222515No Hit
GTATAGGAGGGTCCAACTTTCTTGCACATATAACCATCATATTCAAGTCCAGCTTGAATGCGGCTCAATATACCC4330.14020431622063562No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG4240.13729013874722748No Hit
ATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAGAGCC4080.1321093787945019No Hit
GTACAGGCTATTGAGAATTTGCGTGTCCCTTTCATAGATTTTCAAGGTCCAAGGAGAGGGTACCGACACAGCAAT4050.13113798630336587No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4020.13016659381222984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTTGGACCTTGAAAATCTATGAAAGGGACACGCAAATTCTCAATAGCCTGTACCGAATCCGCAGCAGGTCTC3780.12239545388314148No Hit
GTTCTGAATTGACTGTTAATTGACAACCTTTTAGGCACGCACGCCTTGCGACACACGCACCATTACAGACTTAAT3600.11656709893632523No Hit
GTGTATGGGTTAAAGGTTAATGACATCTCGGTAACGAGAAGCTCTCATAGAGAAGCCAATGGGAGGTATCGGAAG3530.1143005164570078No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT3370.10911975650428221No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.10750076901905549No Hit
CTGTTATACCATATCTCTCTATGAAAGACTTCCATGGTGCATACGATTGTAAAACAGAAAAGAAAACTCTTCCGA3220.104262794048602No Hit
CACCATTACAGACTTAATATACATTCACCGTGAAGCGAATGCATATTGTAAAGCCCGAGCTGCCACAGTCTTCAG3210.10393899655155665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)