FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213586
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.2837264614721939No Hit
GTGCTGGAAGGCGCCGGCCGTGAGGCTAGTGCCAACCTGCTCCCAGGGCTTCTGCTCAAGGCCCAACGCACCCAA4390.20553781614899855No Hit
GTTCAAGAGCGCATGGTGGATGCCTTGGCATCAGGAGCCGATGAAGGACGTTGTAACCTGCGATAAGCCTCGGGG4070.19055556075772756No Hit
GTTCTAAGTTGGTTGTTAAGCGTGCGCCGGACGGGCGAACCCTGCCAAGAGCGTCCGCCACCGGGTGCTGGAAGG3620.16948676411375277No Hit
CCTTTGTGCTGTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTA3480.16293202738007173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.15356811776052737No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3130.1465451855458691Clontech Universal Primer Mix Long (96% over 26bp)
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTGCGTTG3100.14514059910293747No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTAGAGCTGCATT3090.14467240362196024No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860.13390390755948425No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCAGGTAGAGCAGCACACCTTTGTGCTGTCC2670.1250081934209171No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGA2660.12453999793993989No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT2660.12453999793993989No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG2640.12360360697798545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCATTTTCTGGCCATCGCAATGCTATGTTTT2620.12266721601603102No Hit
TTCCAAGGGCGCATGGTGGATGCCTTGGCAACAGGAGCCGAAGAAGGACGTGGGAATCTGCGATAAGCCTGGTGG2480.11611247928234997No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTCGCGTGAAAATCATTCCTTCCGGCGG2470.11564428380137276No Hit
GTACTGATCCGCTATCGGTCAACAGGTAGTATTCAGGCTTACCAGGTGGTCCTGGCAGATTCACACGGGATTCCT2280.10674856966280562No Hit
ATCCTAAGCCATAGGGAAACTCCGTTTTAAAGTGCGCTCTCGGGCGCCGCCTGGCGAAAGGGAAACCGGTTAATA2230.10440759225791953No Hit
GCACAAAGGTGTGCTGCTCTACCTGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGATAGACAGTTA2230.10440759225791953No Hit
ATTCAGACTCGCTTTCGCTACGGCTTCCCCACACGGGTTAACCTCGCCACGTATCACTAACTCGCAGGCTCATTC2160.101130223891079No Hit
GTCCGGTGCGCTCTCGACGACCCTTGAAAATCCGCCGGAAGGAATGATTTTCACGCGAGGTCGTACTCATAACCG2160.101130223891079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)