FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423248
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACG127273.0069840849809095No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG62761.482818583903527Clontech Universal Primer Mix Long (96% over 26bp)
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGG51321.21252787963558No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG49791.1763788606207237Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGC46001.0868332514270593No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT37430.8843514913242354No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACT36410.8602521453143311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35330.8347351907156088No Hit
GCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAG32450.7666899784523494No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG29280.6917929913431369No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG29200.6899028465580463No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT28830.6811609269270026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTAC26690.6305995539258308No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAA23360.551922277246437No Hit
GTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGT22470.5308944165123048No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA21440.5065588024042641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21330.5039598533247647No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT21070.49781688277322045No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTA21050.49734434657694776No Hit
GTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAAC20120.47537141345027034No Hit
AAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGT19440.4593051827770007No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTT19380.4578875741881828No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCG19220.45410728461800176No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19080.45079953124409333Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18450.4359146410615053Clontech Universal Primer Mix Long (96% over 26bp)
GCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGC18200.4300079386080974No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA17710.41843080179941783Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTT17280.4082712735795562No Hit
CTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGG15750.37212225456469966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.35511095149888483No Hit
GATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTC14490.3423524741995237No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG14360.3392809889237516Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCG14280.337390844138661No Hit
CCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCATAACCGCAGCAGGTCT13610.32156088156352775No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC13270.313527766226893Clontech Universal Primer Mix Long (96% over 26bp)
CCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGGCTTCGGCCGTCCGGC13010.30738479567534877No Hit
GGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGC12810.3026594337126224No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12630.2984066079461687Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG12530.2960439269648055Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC12460.2943900502778513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT12310.2908460288058065TruSeq Adapter, Index 7 (96% over 27bp)
ATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCC12140.28682947113748913No Hit
TAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGC12090.28564813064680755No Hit
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT11660.2754886024269459No Hit
GGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTT11400.26934563187540167No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGAC11130.26296639322572113No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG11120.26273012512758476Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGG10920.25800476316485843No Hit
ACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGG10870.25682342267417685No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGG10360.24477374966922466No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.23579556194004458No Hit
GTACATGGGACTGTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTA9910.23414168525309037No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTC9790.23130646807545457No Hit
GATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGGCTTCGGCCGT9500.22445469322950137No Hit
CTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTAT9280.2192567950705024No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATATACCTCCCATTTTGAGCAGCATT9030.21335009261709445No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC8860.20933353494877707No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8650.2043719048879144No Hit
GCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGT8620.20366310059350545No Hit
GGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTT8600.20319056439723282No Hit
CGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTG8580.2027180282009602No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8400.1984652024345065Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8190.19350357237364382Clontech Universal Primer Mix Long (96% over 26bp)
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTAGCTGGA7390.17460212452273843No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGT7350.17365705213019317No Hit
GTATCAACGCAGAGTACATGGGACTGTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAAT7200.1701130306581484No Hit
GGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCATAACC7200.1701130306581484No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAA7030.16609647298983102No Hit
GGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAAC7020.1658602048916947No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA6970.16467886440101312No Hit
GTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCAT6800.16066230673269574No Hit
ACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATG6780.16018977053642308No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6650.15711828526065097Clontech Universal Primer Mix Long (96% over 25bp)
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA6630.15664574906437834No Hit
GCTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA6410.15144785090537935No Hit
GGCTTCGGCCGTCCGGCGTACGGTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATT6250.14766756133519826No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6230.14719502513892566Clontech Universal Primer Mix Long (96% over 26bp)
GGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTC6220.14695875704078934No Hit
GTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTC6220.14695875704078934No Hit
CTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGG6210.146722488942653No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT6130.14483234415756246Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC6080.14365100366688088Clontech Universal Primer Mix Long (96% over 26bp)
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCT6000.14176085888179035No Hit
TACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTG6000.14176085888179035No Hit
CCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGC5890.13916190980229085No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGG5390.127348504895475No Hit
CAGCTAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAG5270.12451328771783919No Hit
CTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA5220.12333194722715761No Hit
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA5160.12191433863833971No Hit
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5140.12144180244206706No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC5080.12002419385324917Clontech Universal Primer Mix Long (96% over 26bp)
GGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAAT5050.1193153895588402No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTAGCTGGAGAATTATTCCCTCCGGTGG4960.11718897667561336No Hit
CCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAAC4940.11671644047934071No Hit
GGCTAGTGGCGATCTGCTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC4880.11529883189052281No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4840.11435375949797755Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4790.11317241900729595Clontech Universal Primer Mix Long (96% over 26bp)
GTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCT4760.11246361471288702No Hit
GGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTT4720.11151854232034172No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4700.1110460061240691Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGG4510.10655691225947908No Hit
GAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTG4420.10443049937625222No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA4410.10419423127811589Clontech Universal Primer Mix Long (96% over 26bp)
GGCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCC4400.1039579631799796No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG4370.10324915888557064Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGACTGTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCT4360.10301289078743431No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.10183155029675273No Hit
TGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCGAGCC4300.10159528219861641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)