FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423248
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACG113422.67975276906211No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGG48751.1518069784145466No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG45371.0719483612444713Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGC41820.9880731864060787No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG36740.8680489925528296Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTT33510.7917343968547991No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACT32420.7659811741579405No Hit
GCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAG29770.7033701281518164No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTAC25660.60626393981779No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTG25630.6055551355233811No Hit
ACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAG25490.6022473821494727No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT22710.5365648508675764Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAA22570.533257097493668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.5278229312365327No Hit
GTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGT20880.49332778890863044No Hit
TATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTA20780.49096510792726716No Hit
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCT20290.47938797111858766No Hit
GTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCA20240.4782066306279061No Hit
GTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAAC19330.45670623369750124No Hit
AAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGT18800.4441840244962764No Hit
CTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTT18330.4330794238838695No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCG17660.41724946130873625No Hit
GCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGC15940.37661134842928967No Hit
GGTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTT15540.36716062450383696No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG15480.3657430159150191Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14310.33809964843307Clontech Universal Primer Mix Long (96% over 26bp)
CTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGG13830.32675877972252676No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13560.32037954107284616Clontech Universal Primer Mix Long (96% over 26bp)
GATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTC13460.318016860091483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.31022001285298456No Hit
GCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCG13000.3071485275772124No Hit
AGTATCACTATCTGTCTCTTATACACATCTGACGCTGCCGACGACATGATGAGTGTAGATCTCGGTGGTCGCCGT12570.29698899935735074Illumina Single End PCR Primer 1 (96% over 27bp)
CCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCATAACCGCAGCAGGTCT12260.28966468831512493No Hit
GGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGC11780.2783238196045817No Hit
TAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGC11560.27312592144558273No Hit
CCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGGCTTCGGCCGTCCGGC11350.2681642913847201No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11090.2620213208331758Clontech Universal Primer Mix Long (96% over 26bp)
ATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCC10990.25965863985181264No Hit
GGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTT10400.24571882206176993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10370.245010017767361Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTT10370.245010017767361No Hit
GCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGAC10070.23792197482327146No Hit
ACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGG9910.23414168525309037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.23130646807545457No Hit
GGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGG9700.2291800551922277No Hit
ATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGG9630.22752617850527349No Hit
GTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTC9350.22091067175745663No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC9340.22067440365932028Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9150.21618530979473027Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9140.21594904169659396Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9060.2140588969115034Clontech Universal Primer Mix Long (96% over 26bp)
GCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGT8850.20909726685064078No Hit
GTACATGGGACTGTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTA8460.1998828110233244No Hit
CTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTAT8430.19917400672891544No Hit
TCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCG8430.19917400672891544No Hit
GGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTT8400.1984652024345065No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCAGTATTTAGCTTTAGAAGAAATATACCTCCCATTTTGAGCAGCATT7980.18854194231278115No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCC7840.18523418893887272No Hit
GATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGGCTTCGGCCGT7810.18452538464446377No Hit
CGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTG7530.1779098778966469No Hit
GAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAATTAGACAGT7310.1727119797376479No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTAGCTGGA6990.16515140059728575No Hit
GTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCAT6730.1590084300457415No Hit
ATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGCTATGTTTTAA6700.15829962575133255No Hit
GGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGGTCGTACTCATAACC6620.156409480966242No Hit
GTATCAACGCAGAGTACATGGGACTGTCGGGTCTGGTGCGCCCCTGACGTCCCTTGAAAATCCACCGGAGGGAAT6500.1535742637886062No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6480.1531017275923336Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATG6380.1507390466109704No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6340.14979397421842514Clontech Universal Primer Mix Long (96% over 26bp)
GGCTTCGGCCGTCCGGCGTACGGTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATT6270.14814009753147092No Hit
GGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAAC6180.14601368464824405No Hit
ACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAA6090.1438872717650172No Hit
GAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTA6070.14341473556874457No Hit
GTTAACAACCAACTTAGAACTGGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTC6060.14317846747060825No Hit
GCTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTA5930.1401069821948361No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG5430.12829357728802027Clontech Universal Primer Mix Long (96% over 25bp)
TACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTG5400.12758477299361132No Hit
GATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCT5380.12711223679733868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200.12285941103088496No Hit
GGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTC5200.12285941103088496No Hit
CCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGC5130.12120553434393075No Hit
CTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTTCAGCACCTGGGTTCTGACGGTTTTGGCAGG5070.11978792575511284No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTAGCTGGAGAATTATTCCCTCCGGTGG5070.11978792575511284No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5070.11978792575511284Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5050.1193153895588402Clontech SMART CDS Primer II A (96% over 26bp)
CCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCTTATCTACATTGTTCTATCAAC5000.11813404906815862No Hit
CTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAA4990.11789778097002229No Hit
GGTACGGACAGGGGGAATCTGACTGTCTAATTAAAACATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAAT4970.11742524477374967No Hit
CCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAAC4850.11459002759611385No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4840.11435375949797755Clontech Universal Primer Mix Long (96% over 26bp)
CTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAA4770.11269988281102333No Hit
CAGCTAGGTCGTACTCATAACCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGTTGATAGAACAATGTAGATAAG4590.10844705704456963No Hit
AAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGGGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGG4540.10726571655388802No Hit
GGTTGGGTACGTTGGGCCTTTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGCGGCTTT4410.10419423127811589No Hit
GGCTAGTGGCGATCTGCTCCCGAGCCGTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCC4380.10348542698370695No Hit
TGCCAAAACCGTCAGAACCCAGGTGCTGAAAGCCGCCGGCCGGTTGCCCGGCTAGTGGCGATCTGCTCCCGAGCC4310.10183155029675273No Hit
GTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCGAAGTTACGGATCTATTTTGCCGACTTCCCT4240.10017767360979851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)