FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257850
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG32321.2534419235989918Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26841.0409152608105487Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT13880.5382974597634284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.38084157455885204No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9560.37075819274772154Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG8800.34128369206903236Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7860.30482838859802214Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA7690.29823540818305216Clontech Universal Primer Mix Long (96% over 26bp)
CTACAGAGGTGTAGCGCTCTGCCGGCCATTAAACCCTAGCTCCGGACAAACCGATTTCAGGGTGACAGACCGTTA7200.2792321116928447No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC6900.2675974403723095Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGGGTTACCGCAGCGAATGAGATTTCGCTACCATTACCTAACGAGGAATACACTGAACCAGCTCACCAGGA6880.26682179561760716No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6540.25363583478766727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT6360.24665503199534614Clontech Universal Primer Mix Long (96% over 26bp)
ATAGTGATACTCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCAGGATCATCTCGTATGCCGTCTTCTGCT5800.22493697886368044TruSeq Adapter, Index 7 (96% over 27bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG5780.2241613341089781Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTTTATAGTGCTCAATAACACTCCTGCGTTACGGTAGTCGTACCGGTTTAGCAGAGTTTTGTAGACTATAAAG5540.21485359705254994No Hit
CCTCTGTAGCGTCCGGTGCGCTCCCGACGTCCCTTGAAAATCCGCTGGAAGGAATAAGATTCGCGCCAGGTCGTA5390.20903626139228232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5240.20321892573201472No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG4960.1923598991661819Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAGGAGTGTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCA4370.16947837890246267No Hit
CTCCTAAGCGGATTCCGACTTCCATGGCCACCGTCCGGCTGTCTGGATGCATTGACACCTTTTGTGGTGTCTGAT4340.16831491177040916No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC4310.16715144463835563Clontech Universal Primer Mix Long (96% over 26bp)
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGTATGTGAAATTGTTGAAAGGGAAG4250.16482451037424858No Hit
CTATAAAGATAGCGCAATGTAACCGCATTACGCCCTAGTGCAACCATTGCGAAGTTGGGAACTGTAGGTAGTCAT4120.15978281946868333No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4110.15939499709133217No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC3910.1516385495443087Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG3910.1516385495443087Clontech Universal Primer Mix Long (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3890.15086290478960637Clontech SMART CDS Primer II A (96% over 26bp)
CTCTACTTGTGCGCTATCGGTCTCGGGCCAATATTTAGCTTTAGAAGAAATTTACCTCCCATTTTGACCTGCATT3310.12836920690323833No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3300.12798138452588714Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAAGGGCGTCCACACCAGGTGCTGGGACCC3190.12371533837502424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.11789800271475663No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGCTGTTGGGACCCGAAAGATGGTGAACTATGCGTGAAT2890.11208066705448905No Hit
GTGTAACAACTCACCGGCCGAATGCATTGGCCCTGAAAATGGATGGCGCTTAAGCGTGTTACCCATACCTCGCCG2840.11014155516773318No Hit
GTATGGTACTGACTATGAATCATACTGCAAACATTACCTTCTTTATCTTCAAGTTGGTTCCGCCACTGGGCGAGA2820.10936591041303084No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2810.10897808803567967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGA2800.10859026565832848No Hit
ACCCTAGGGCTTAGGATCGACTCACCCATGTCCAACTGCTGTTCACATGGAACCTTTCCCCACTTCAGTCCTCAA2740.10626333139422145No Hit
CTTGTGCGCCGTCGGGCGAAAGGGAAGCCGGTTAATATTCCGGCACCTGGATGCGGTTTCTTCACGGCAACGTAA2740.10626333139422145No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTGGCGCGAATCTTATTCCTTCCAGCGG2740.10626333139422145No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC2730.10587550901687028Clontech Universal Primer Mix Long (96% over 26bp)
GGTTAAGGGTGTTTCACCCCCAAGGCCTCTAATCATTCGCTTTACCTCATAAAACTGATTTTTCGTTACTGCTAT2590.10044599573395385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)