FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248461
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG38671.5563810819404251Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG33961.3668141076466729Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21770.8761938493365157Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.7542431206507259No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG16060.6463791097999284Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC14940.6013016127279532Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14290.5751405653201106Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14020.5642736687045452Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA13280.5344903224248474Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11280.45399479193917763No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC9640.3879884569409284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG9430.37953642623993306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG9310.37470669441079285Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT9310.37470669441079285Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC9270.37309678380107947Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACG8550.3441183928262383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.3199697336805374No Hit
ATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAA7190.2893814320959829No Hit
GCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAACACCGTTTATCCCTCTA7170.2885764767911262No Hit
CCATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAA7050.283746744961986No Hit
GTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTTCGGCCGC6880.27690462487070405No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG6740.2712699377367072Clontech Universal Primer Mix Long (96% over 25bp)
CCACTAGGTTGCCCCAACAACACGCCAAATTGCCAGTTCTCTCTGGACGAACCTCGAACAAGCTCTCTGCACACG6040.24309650206672273No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6030.24269402441429438Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6000.24148659145700938Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTT5890.2370593372802975No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT5460.2197527982258785Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.20244625917145953No Hit
TATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGA4830.19439670612289253No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC4460.17950503298304363Clontech Universal Primer Mix Long (96% over 26bp)
TCTTAGAGGGATAAACGGTGTTTTAGCCGTAAGAAACGAGGCAATAACAGGTCTGTGATGCCCTTAGATATTCTA4340.17467530115390342No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG4290.1726629128917617Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4270.171857957586905Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC4040.16260097158105297Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG3980.16018610566648286Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG3790.15253903027034424Clontech Universal Primer Mix Long (96% over 26bp)
CAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTTCGGCCGCGGTTTCTCTTAG3760.15133159731305917No Hit
AGCCTAGACGGCGCGAATGGCTCTGCCAAGATTGACTGCGGCTGCCCTCAGGTTAGAGGTGGCAACATCTCTAGC3720.1497216867033458No Hit
GTGGTGCATGGCCGTTCTTGATTCGTATTCCGAAACGTCTGCTTAATTGCGATAACGAGTGAGACCTCACCCAAC3660.14730682078877572No Hit
CGCCATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCT3580.1440869995693489No Hit
GTCTAGGCTCGTTCAGCTTTTTGACGACTTCACTTGCCAGGTCAGCACAAACAGAGCTCATAACCAAAATCAAAC3530.14207461130720717No Hit
GTGCATGGCCGTTCTTGATTCGTATTCCGAAACGTCTGCTTAATTGCGATAACGAGTGAGACCTCACCCAACTAA3440.13845231243535203No Hit
ATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAACACCGTTTATCCCTCTAAGAAGTCCG3440.13845231243535203No Hit
CTCTTAGCTCGCTAACCAAAGTCACTTAACAATCGACTTATAAGCTAGGTTGCCCCAACTCACAAACCAAACTGC3380.13603744652078192No Hit
CCCTTAGATATTCTAGGCCGCACGCGTGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGA3280.13201266999649844No Hit
ATTCTAGGCCGCACGCGTGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAGT3160.12718293816735826No Hit
TGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAGTTTGGTTTGTGAGTTGGG3150.12678046051492992No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT3080.12396311694793147Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCAC3050.12275568399064642No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA3050.12275568399064642Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT3040.12235320633821806Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG2980.11993834042364798Clontech Universal Primer Mix Long (96% over 26bp)
CCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTTCGGCCGCGGTTTCTCTTAGCTCGCTAACC2980.11993834042364798No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA2960.11913338511879129Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2900.11671851920422119No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2880.11591356389936448Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2880.11591356389936448Clontech Universal Primer Mix Long (96% over 26bp)
TGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAATTTGGCGTGTTGTTGGGG2830.11390117563722273No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT2780.111888787375081Clontech Universal Primer Mix Long (96% over 26bp)
AAGCTAGGTTGCCCCAACTCACAAACCAAACTGCCAGTTCTCTCTGGACGAACCTCGAACAAGCTCTCTGCACAC2770.11148630972265265No Hit
GTGTATTTCTGATTGCCACGACCTTTCGGCCGCGGTTTCTCTTAGCTCGCTAACCAAAGTCACTTAACAATCGAC2750.11068135441779595No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2650.10665657789351245No Hit
ATTCTAGGCCGCACGCGTGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAAT2610.10504666728379908No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC2530.10182684606437227Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT2530.10182684606437227Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)