FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002148975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002148975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248461
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG28211.1353894575003722Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG22840.919258958146349Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT16110.6483914980620701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12670.509939185626718No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11830.4761310628227368Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11520.46365425559745793Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG11090.44634771654303895Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10340.41616189261091274Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9050.3642422754476558Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7740.31151770297954207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7720.3107127476746854No Hit
GTATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACG7590.3054805381931168No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG7170.2885764767911262Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT7060.28414922261441433Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC6840.27529471426099067Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC6790.27328232599884894Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAACACCGTTTATCCCTCTA5950.2394742031948676No Hit
ATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAA5780.23263208310358566No Hit
GTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTTCGGCCGC5680.22860730657930217No Hit
CCACTAGGTTGCCCCAACAACACGCCAAATTGCCAGTTCTCTCTGGACGAACCTCGAACAAGCTCTCTGCACACG5670.22820482892687383No Hit
CCATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAA5500.2213627088355919No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG5450.21935032057345016Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTT5360.215728021701595No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.2141181110918816No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4620.18594467542189722Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4410.17749264472090187Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGA4350.17507777880633177No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC4330.17427282350147508Clontech Universal Primer Mix Long (96% over 26bp)
TCTTAGAGGGATAAACGGTGTTTTAGCCGTAAGAAACGAGGCAATAACAGGTCTGTGATGCCCTTAGATATTCTA3750.15092911966063083No Hit
ATTCTAGGCCGCACGCGTGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAGT3710.14931920905091745No Hit
GTGGTGCATGGCCGTTCTTGATTCGTATTCCGAAACGTCTGCTTAATTGCGATAACGAGTGAGACCTCACCCAAC3680.1481117760936324No Hit
GTCTAGGCTCGTTCAGCTTTTTGACGACTTCACTTGCCAGGTCAGCACAAACAGAGCTCATAACCAAAATCAAAC3540.1424770889596355No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC3450.13885479008778037Clontech Universal Primer Mix Long (96% over 26bp)
AGCCTAGACGGCGCGAATGGCTCTGCCAAGATTGACTGCGGCTGCCCTCAGGTTAGAGGTGGCAACATCTCTAGC3270.1316101923440701No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3250.1308052370392134Clontech Universal Primer Mix Long (96% over 26bp)
GTGCATGGCCGTTCTTGATTCGTATTCCGAAACGTCTGCTTAATTGCGATAACGAGTGAGACCTCACCCAACTAA3160.12718293816735826No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.12678046051492992No Hit
CAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCACGACCTTTCGGCCGCGGTTTCTCTTAG3130.12597550521007322No Hit
CGCCATTGTAGCACGCGTGCGGCCTAGAATATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCT2980.11993834042364798No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC2960.11913338511879129Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAAGGGCATCACAGACCTGTTATTGCCTCGTTTCTTACGGCTAAAACACCGTTTATCCCTCTAAGAAGTCCG2930.11792595216150624No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG2920.11752347450907788Clontech Universal Primer Mix Long (96% over 26bp)
TGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGAACTGGCAGTTTGGTTTGTGAGTTGGG2920.11752347450907788No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2840.11430365328965109Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGCAACAATTGCAATCCCCAATCCCAAATCACGGCGTGTATTTCTGATTGCCAC2820.11349869798479441No Hit
CTCTTAGCTCGCTAACCAAAGTCACTTAACAATCGACTTATAAGCTAGGTTGCCCCAACTCACAAACCAAACTGC2790.11229126502750936No Hit
CCCTTAGATATTCTAGGCCGCACGCGTGCTACAATGGCGTGTGCAGAGAGCTTGTTCGAGGTTCGTCCAGAGAGA2770.11148630972265265No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG2550.10263180136922898Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)