FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences530919
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.5522499665674048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.33131230941066336No Hit
CCCCTGGTCCGTGCCAGTTCTGAGTTGATCGTTGAATGGCGACCGCGGGGGTAGAAGGCCCGCCTACGGGGCGCC17090.3218946769657895No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGTGGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA16390.3087099915429661TruSeq Adapter, Index 25 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.19419158101329959No Hit
CTCTATAAGCGTTCGAGTTCCCTGGAATCCTTTAGCAGGGAGATAGGGTTTGGAATGCGAAGAGCATCGCAGTTG9300.17516796347465433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8380.15783951977608637No Hit
GTCCCGTGCCTTGGCCTCACGCGGATCTTCCTTGCTGTGAGGTGCTGGCTGTCAGCCGCGGGATACGCTCCTCAC8100.15256564560695698No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.13862754958854365No Hit
GCGTAGACGTGCCGGGAGCCGGGCCTGGGCGAGTCGATCCGTGTGCGTGGGAGCAATCCCCGCCTCGGTGATTCT7300.13749743369515877No Hit
CTTATAGAGAAAAGAAAACTCTCTCCGGATCTCCCGACGGCGTCTCCGGGTCTCTTTGGGTTACCCCGACGAGCA7300.13749743369515877No Hit
GCACGGGACCGAGCAGAGAATCACCGAGGCGGGGATTGCTCCCACGCACACGGATCGACTCGCCCAGGCCCGGCT7070.1331653227705168No Hit
GTTCTGAGTTGATCGTTGAATGGCGACCGCGGGGGTAGAAGGCCCGCCTACGGGGCGCCCCGTGAGGAGCGTATC6870.12939826979256722No Hit
GTATCGGGCTTGGTCGGGAAGCGTAGACGTGCCGGGAGCCGGGCCTGGGCGAGTCGATCCGTGTGCGTGGGAGCA6810.12826815389918236No Hit
GTGTGCGTGGGAGCAATCCCCGCCTCGGTGATTCTCTGCTCGGTCCCGTGCCTTGGCCTCACGCGGATCTTCCTT6480.12205251648556559No Hit
GGGGTAGAAGGCCCGCCTACGGGGCGCCCCGTGAGGAGCGTATCCCGCGGCTGACAGCCAGCACCTCACAGCAAG6420.12092240059218073No Hit
ATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGTGTATC6270.11809711085871855No Hit
GAATAATGTAGGTAAGGGAAGTCGGCAAACTTGATCCGTAACTTCGGAACAAGGATTGGCTCTGAGGATCGGGGT5590.10528913073369008No Hit
GTCCGGATCTTCCCCTCGGACCTTGAAAATCCGGGAGAGGGCTACGTGGAAAGTTCGCACCGGATCGTACCCATA5430.10227548835133042No Hit
CCTACGGGGCGCCCCGTGAGGAGCGTATCCCGCGGCTGACAGCCAGCACCTCACAGCAAGGAAGATCCGCGTGAG5320.10020360921345817No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)