FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281422
Sequences flagged as poor quality0
Sequence length75
%GC25

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73432.6092487438792986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48051.707400274321126No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36521.2976952761333513No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA30921.0987058581063314Illumina PCR Primer Index 12 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23920.8499690855725566No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.38092260022315244No Hit
ATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA9350.3322412604558279Illumina PCR Primer Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA8990.31944908358266233Illumina PCR Primer Index 12 (96% over 29bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8900.31625103936437093No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.2867579649067948No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.27147842030829145No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.1350285336611921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3660.1300537982105166No Hit
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3640.12934312171756296No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3530.12543440100631792No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.1218810185415497No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3280.11655094484439739No Hit
TATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3190.11335290062610599Illumina PCR Primer Index 12 (96% over 29bp)
ACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA3190.11335290062610599Illumina PCR Primer Index 12 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTT2910.103403429724755No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)