FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148288
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29692.0021849374190763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18311.2347593871385412No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA16611.1201176089771256Illumina PCR Primer Index 12 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13550.9137624082865775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.6291810530858869No Hit
ATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5050.34055351747949936Illumina PCR Primer Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA5020.33853042727665084Illumina PCR Primer Index 12 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.3081840742339232No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.23535282693137677No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.20433211048769961No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.19691411307725507No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG2280.15375485541648684No Hit
TATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1860.1254315925766077Illumina PCR Primer Index 12 (96% over 29bp)
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.1099212343547691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1600.10789814415192059No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)