FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115336
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84717.344627869875842No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52854.5822639938960945No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA39133.392696122633003Illumina PCR Primer Index 12 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33782.928834015398488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32012.775369355621835No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23922.0739404869251574No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20691.7938891586321706No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16031.389852257751266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10780.9346604702781438No Hit
ATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA10040.8705001040438372Illumina PCR Primer Index 12 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA9630.8349517930221267Illumina PCR Primer Index 12 (96% over 29bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.6754179094124991No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.5843795519178747No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.35808420614552267No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3980.34507872650343346No Hit
TATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA3750.32513699105223Illumina PCR Primer Index 12 (96% over 29bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.3242699590760907No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2920.25317333703266975No Hit
ACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA2610.22629534577235208Illumina PCR Primer Index 12 (96% over 29bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.18988000277450232No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2120.18381077894152736Illumina PCR Primer Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA2030.17600749115627384Illumina PCR Primer Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC2010.17427342720399527Illumina PCR Primer Index 12 (96% over 29bp)
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.16733717139488102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.1604009155857668Clontech SMART CDS Primer II A (100% over 21bp)
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.15346465977665258No Hit
GGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1720.14912949989595614No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA1610.13959214815842408TruSeq Adapter, Index 5 (96% over 27bp)
TCCCTAGACCGTGCCAGTTCTGAATTGATTGTTAATTGATAACTCACACCCTTTAGGAAGGGCCGTTGCCAGCCC1490.12918776444475275No Hit
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG1460.12658666851633488Illumina PCR Primer Index 12 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1460.12658666851633488Illumina PCR Primer Index 12 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA1270.11011306096968858Illumina PCR Primer Index 12 (96% over 29bp)
CAGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.10751196504127072No Hit
CAGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.10317680516057433No Hit
CAGTTATACACATCTCCGAGCCCACGAGACCTATTGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1170.10144274120829576Illumina PCR Primer Index 12 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)