FastQCFastQC Report
Wed 8 May 2024
EGAF00002149059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277193
Total Bases20.7 Mbp
Sequences flagged as poor quality0
Sequence length75
%GC31

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69362.5022276897324245No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA66012.3813732670016923No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49641.7908100132398728No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44041.588784709570588No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42551.5350315484157246No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA28901.0425948707218435No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA26890.9700822170834039No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24720.8917974119115561No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA22780.8218100745689827No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA20850.752183496697247No Hit
ATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20100.7251265363843964No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18020.6500885664500907No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA16390.5912847727034953No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16190.5840695832867352No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15570.5617024960947787No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12660.45672149008091834No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11260.40621516416359715No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9900.35715187612962807No Hit
GTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9500.3427214972961078No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA9480.34199997835443174No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA9260.33406326999599556No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.30664555021230694No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7470.26948732471599207No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA7260.2619113758283939No Hit
GTATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAA7040.2539746674699578No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6940.25036707276157766No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA6770.2442341617573315No Hit
TCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6650.23990504810727542No Hit
GTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6450.23268985869051528No Hit
ACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6390.2305253018654872No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC6350.2290822639821352No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA6320.22799998556962117No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA6100.220063277211185No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA5930.21393036620693884No Hit
TATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5730.2067151767901787No Hit
GTATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAA5430.19589239266503844No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5400.1948101142525244No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA5300.19120251954414433No Hit
TATCAACGCAGAGTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA5250.1893987221899543No Hit
GGAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4950.17857593806481403No Hit
TATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA4820.17388606494391992No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA4760.1717215081188919No Hit
ACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4670.16847467288134982No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC4210.15187973722280143No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA4180.15079745881028742No Hit
TATCAACGCAGAGTACATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAA4150.1497151803977734No Hit
CAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4070.14682910463106932No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA3980.14358226939352725No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA3890.14033543415598518No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA3860.13925315574347114No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA3720.13420252315173906No Hit
AACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3650.13167720685587297No Hit
AACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3610.13023416897252094No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3510.12662657426414087No Hit
TCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAA3440.12410125796827481No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.11580379013900062No Hit
GTATCAACGCAGAGAACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.11327847384313458No Hit
CAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA3120.11255695490145853No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.11111391701810651No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCC3060.1103923980764305No Hit
ACGCAGAGTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAAAAAAAA2980.10750632230972644No Hit
GTATCAACGCAGAGTACATGGGAAGCACTGGTATCAACGCAAAAAAAAAA2900.10462024654302236No Hit
GAATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2890.10425948707218435No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2860.10317720865967034No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA2790.10065189236380429No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)