FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157692
Sequences flagged as poor quality0
Sequence length75
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50293.189128173908632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31642.0064429394008574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17701.122441214519443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.929026202977957No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA14010.8884407579331862Illumina PCR Primer Index 9 (96% over 28bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.5504400984197042No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.45341551885954895No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.3640007102452883No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5070.32151282246404383No Hit
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4310.27331760647337844Illumina PCR Primer Index 9 (96% over 28bp)
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.14078076249904878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT2140.13570758186845241No Hit
TTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2130.13507343428962787Illumina PCR Primer Index 9 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT1950.1236587778707861No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.1236587778707861No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1880.11921974481901429No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)