FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193992
Sequences flagged as poor quality0
Sequence length75
%GC34

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA97435.022372056579653Illumina PCR Primer Index 9 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA31481.6227473297867951Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA27311.4077900119592561Illumina PCR Primer Index 9 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15670.8077652686708731No Hit
TATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA10120.5216709967421337Illumina PCR Primer Index 9 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.46032826095921475No Hit
ACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA8340.4299146356550786Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA7180.3701183553960988Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA7180.3701183553960988Illumina PCR Primer Index 9 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.3665099591735742No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC5650.29124912367520306Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA4660.24021609138521177Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG4610.23763866551197985Illumina PCR Primer Index 9 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.21650377335147838No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA3880.20000824776279436TruSeq Adapter, Index 9 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA3810.19639985154026968Illumina PCR Primer Index 9 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACA2670.1376345416305827Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA2610.13454163058270444Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG2300.11856159016866676Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA2260.11649964947008123Illumina PCR Primer Index 9 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA2230.1149531939461421Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA2150.11082931254897109Illumina PCR Primer Index 9 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA2080.10722091632644644Illumina PCR Primer Index 9 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAC2060.10618994597715371Illumina PCR Primer Index 9 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA2030.10464349045321457Illumina Single End Adapter 1 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)