FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503791
Sequences flagged as poor quality0
Sequence length75
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84991.6870090970263465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50681.0059727148758115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.8259377400549037No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.4462167843411256No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGGCCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA8780.17427861950689869Illumina PCR Primer Index 9 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.1407329626769831No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.13378563729800652No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)