FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171961
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42742.485447281651072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25571.4869650676606905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20251.1775925936694949No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA15980.9292804763870878TruSeq Adapter, Index 18 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12160.7071370834084473No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.3303074534342089No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4870.2832037496874291No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4320.2512197533161589No Hit
ATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4130.24017073638790193TruSeq Adapter, Index 18 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA3570.20760521280988128RNA PCR Primer, Index 30 (96% over 29bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.20178994074237763No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT2000.11630544135007356No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)