FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230397
Sequences flagged as poor quality0
Sequence length75
%GC28

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52112.2617481998463522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31531.368507402440136No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23561.0225827593241232No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA18210.7903748746728473TruSeq Adapter, Index 18 (96% over 28bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14510.6297825058486004No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.31293810249265397No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5420.23524611865605888No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5370.23307595150978527No Hit
ATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4850.2105062131885398TruSeq Adapter, Index 18 (96% over 28bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.18967260858431317No Hit
TTATACACATCTCCGAGCCCACGAGACGTAACCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4150.1801238731407093RNA PCR Primer, Index 30 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)