FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686713
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG98521.4346604767930708Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG79591.1589994655700417Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48100.700438174317364No Hit
AGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCG46460.6765562906192253No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT35330.5144798482044173Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCA35250.5133148782679227No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCGGTAAGAGACG31010.45157147163371014No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28810.4195347983801093No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC28270.4116712513087709Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG25180.3666742875116679Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA21290.31002762434961917Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGACCCGAAACCGGGCGATCTATCCATGAGCAGGTTGAAGGTTGGGTAACACTAACTGGAGGACCGAACCC20700.30143597106797165No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20700.30143597106797165Clontech Universal Primer Mix Long (96% over 26bp)
GTGCTAACCTGCGAAAAGCTGTGAGAAGTCGGTAAGAGACGTTAGACTCACAGATGTCCGAATGGGGAAACCCAC20400.297067333806117No Hit
GCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCGG20210.29430053020694236No Hit
GGTCTACACCCTGCAACTATTCGCCCAGTTAAGACTCGGTTTCCCTACGGCTCCCCTATTCGGTTAACCTCGCTA19970.2908056203974586No Hit
TTCCAGAGCGTTCCACTAACGCTTTATCAACTTAAGGGCTAGCCCCCGTTCGCTCGCCGCTACTAAGGGGATCTC19240.2801752697269456No Hit
GGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCACCCTGTATCGC19110.27828219358014195No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT17960.26153575074303237Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.21493695328324933No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC14560.21202452844201292Clontech Universal Primer Mix Long (96% over 26bp)
TCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTA14470.21071393726345647No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC14380.20940334608490008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG14060.2047434663389218Clontech Universal Primer Mix Long (96% over 25bp)
ACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAACGTCTC13920.20270476895005626No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAG13480.19629743429933613No Hit
GACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGA13100.19076382710098688No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTC13080.19047258461686323No Hit
ATACATAGTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATC12950.18857950847005955No Hit
GTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATCCCCTTAG12830.18683205356531768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.18610394735500857No Hit
GTGTAATGATGGCCAGGCTGTCTCCACCAGAGACTCAGTGAAATTGAAATTGCGGTGAAGATGCCGTATACCCGC12370.18013347643047387No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT11670.16993998948614633Clontech SMART CDS Primer II A (96% over 26bp)
GTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGG11420.16629945843460076No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG11260.16396951856161163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACT10930.1591640175735715No Hit
ACTTAGATGTTTCAGTTCTCCCGGTTCGCCTCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTG10840.1578534263950151No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10740.15639721397439688Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTAGATGTTTCAGTTCTCCCGGTTCGCCTCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGG10610.1545041378275932No Hit
AGCCTTAACAGTGCTCTACCCCCAATGGTATTCGCTCGAGGCGCTACCTAAATAGCTTTCGAGGAGAACCAGATA10320.15028112180780034No Hit
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCT10280.14969863683955306No Hit
GCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATA10280.14969863683955306No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTT10260.14940739435542943No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10210.1486792881451203Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTC9820.14300005970470925No Hit
GCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCC9700.14125260479996737No Hit
ACCTGAAACCGTGTACGTACAAGCAGTGGGAGCCCCTTCGTGGGGTGACTGCGTACCTTTTGTATAATGGGTCAG9630.14023325610553464No Hit
GTGTCTGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAGTAC9200.13397154269687628No Hit
GTTTAGCCCCGTTACATCTTCCGCGCAGACCGACTCGACTAGTGAGCTATTACGCTTTCTTTAAAGGGTGGCTGC9020.13135036033976347No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGA8980.13076787537151618No Hit
GGGCTATCACCCTGTATCGCCGCACTTTCCAGAGCGTTCCACTAACGCTTTATCAACTTAAGGGCTAGCCCCCGT8890.1294572841929598No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC8870.12916604170883614Clontech Universal Primer Mix Long (96% over 26bp)
GTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAACGTCTCTTACC8610.1253798894152288No Hit
GTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCACCCTGTATCGCCGCACTTTCCA8470.12334119202636326No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG8420.12261308581605415Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGGGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACC8390.12217622208986868No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGTGTAATGATGGCCAGGCTGTCTC8300.12086563091131229No Hit
GTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAAC8270.12042876718512682No Hit
GCTCTGGAAAGTGCGGCGATACAGGGTGATAGCCCCGTACACGAAAAGGCTTTATCAATGAAAACGAGTAGGACG8220.11970066097481771No Hit
GTATGGAAGGGCCATCGCTCAACGGATAAAAGGTACTCCGGGGATAACAGGCTGATACCGCCCAAGAGTTCATAT7920.11533202371296306No Hit
GTGTAGGATAGGTGGGAGACTTTGAAGTTGGAACGCCAGTTCTGATGGAGTCGTCCTTGAAATACCACCCTTGTA7920.11533202371296306No Hit
TGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCAC7840.11416705377646848No Hit
GATGAATACATAGTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCG7710.1122739776296648No Hit
GTATCAACGCAGAGTACATGGGGGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAA7630.11110900769317023No Hit
ACTCACAGATGTCCGAATGGGGAAACCCACCTGATTTATCAGGTATCGTATGATGAATACATAGTCATACGAGGC7580.1103809014828611No Hit
GTGACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGA7480.1089246890622429No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG7410.10790534036781013Clontech Universal Primer Mix Long (96% over 26bp)
TAGTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATCCCCTT7350.1070316129154392No Hit
GTTAAGGCTAGGGGGTCATCCCGACTTACCAACCCTTTGCAAACTCCGAATACCAATGAGTACTACTCGGGAGAC7330.10674037043131555No Hit
GTACATGGGATGACTCATCTTTTGGCCGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCGTTCCGAACATAGCT7220.10513853676863551No Hit
ACTTAAGGGCTAGCCCCCGTTCGCTCGCCGCTACTAAGGGGATCTCGGTTGATTTCTTTTCCTCGGGGTACTTAG7190.10470167304245004No Hit
ACCCTGTATCGCCGCACTTTCCAGAGCGTTCCACTAACGCTTTATCAACTTAAGGGCTAGCCCCCGTTCGCTCGC7170.10441043055832641No Hit
GTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCACCCTGTATCGCCGCACTTTCCAGAGCG7120.1036823243480173No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC7050.10266297565358452Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATATGAACT6950.10120676323296632No Hit
CCCCTTAGTAGCGGCGAGCGAACGGGGGCTAGCCCTTAAGTTGATAAAGCGTTAGTGGAACGCTCTGGAAAGTGC6900.10047865702265721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)