FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002149099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686713
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG67290.9798853378340004Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG52020.7575217012055983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCG42950.6254432346555256No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCA30010.437009347427528No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCGGTAAGAGACG28260.41152563006670906No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT23950.3487628747380638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC19010.2768259811595237Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG18630.27129237396117445Clontech Universal Primer Mix Long (96% over 26bp)
GCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCGG17390.2532353399455085No Hit
TTCCAGAGCGTTCCACTAACGCTTTATCAACTTAAGGGCTAGCCCCCGTTCGCTCGCCGCTACTAAGGGGATCTC17060.2484298389574684No Hit
GGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCACCCTGTATCGC15990.23284836605685344No Hit
GTGCTAACCTGCGAAAAGCTGTGAGAAGTCGGTAAGAGACGTTAGACTCACAGATGTCCGAATGGGGAAACCCAC15700.22862535003706058No Hit
GGTCTACACCCTGCAACTATTCGCCCAGTTAAGACTCGGTTTCCCTACGGCTCCCCTATTCGGTTAACCTCGCTA15600.2271691376164424No Hit
GTGTAGACCCGAAACCGGGCGATCTATCCATGAGCAGGTTGAAGGTTGGGTAACACTAACTGGAGGACCGAACCC15540.22629541016407148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.22090742420778406No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA15140.22047056048159858Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG14400.20969458856902376Clontech Universal Primer Mix Long (96% over 26bp)
TCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTA14110.2054715725492309No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT13680.19920985914057254Clontech Universal Primer Mix Long (96% over 26bp)
GACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGA12430.1810072038828448No Hit
ACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAACGTCTC12320.17940537022016476No Hit
GTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATCCCCTTAG11260.16396951856161163No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTC10800.1572709414267678No Hit
ATACATAGTCATACGAGGCGAACCGGGAGAACTGAAACATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATC10700.1558147290061496No Hit
ACTTAGATGTTTCAGTTCTCCCGGTTCGCCTCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTG10480.1526110616807895No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10400.15144609174429494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGG10370.15100922801810945No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG10350.15071798553398583Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTAGATGTTTCAGTTCTCCCGGTTCGCCTCGTATGACTATGTATTCATCATACGATACCTGATAAATCAGG10010.14576686330388386No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC9870.14372816591501836Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGATACCGCCCAAGAGTTCATATCGACGGCGGTGTTTGGCACCTCGATGTCGGCTCATCACATCCTGGGGCT9330.13586461884367995No Hit
GTCTCACGACGTTCTAAACCCAGCTCGCGTACCACTTTAAATGGCGAACAGCCATACCCTTGGGACCGACTTCAG9300.1354277551174945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9290.13528213387543267Clontech SMART CDS Primer II A (96% over 26bp)
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTT8780.12785545053027975No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTC8550.12450616196285785No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8430.12275870705811599Clontech Universal Primer Mix Long (96% over 26bp)
AGCCTTAACAGTGCTCTACCCCCAATGGTATTCGCTCGAGGCGCTACCTAAATAGCTTTCGAGGAGAACCAGATA8370.12188497960574506No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG8350.1215937371216214Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8330.12130249463749776No Hit
GTGTAATGATGGCCAGGCTGTCTCCACCAGAGACTCAGTGAAATTGAAATTGCGGTGAAGATGCCGTATACCCGC8220.11970066097481771No Hit
GTACATGGGGGTTAAGTGACTAAGCGTACACGGTGGATGCCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACC8170.11897255476450862No Hit
GTATCAGCCTGTTATCCCCGGAGTACCTTTTATCCGTTGAGCGATGGCCCTTCCATACAGAACCACCGGATCACT8120.1182444485541995No Hit
GCCATACCCTTGGGACCGACTTCAGCCCCAGGATGTGATGAGCCGACATCGAGGTGCCAAACACCGCCGTCGATA8100.11795320607007585No Hit
ACCTGAAACCGTGTACGTACAAGCAGTGGGAGCCCCTTCGTGGGGTGACTGCGTACCTTTTGTATAATGGGTCAG8030.1169338573756431No Hit
GCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCC7900.11504078122883941No Hit
GCTCTGGAAAGTGCGGCGATACAGGGTGATAGCCCCGTACACGAAAAGGCTTTATCAATGAAAACGAGTAGGACG7830.11402143253440666No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG7790.11343894756615937Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTATCACCCTGTATCGCCGCACTTTCCAGAGCGTTCCACTAACGCTTTATCAACTTAAGGGCTAGCCCCCGT7770.11314770508203574No Hit
GTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCACCCTGTATCGCCGCACTTTCCA7750.11285646259791209No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGA7620.11096338645110838No Hit
GTGTCTGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAGAGTAACGGAGGAGCACGAAGGTTGGCTAAGTAC7380.10746847664162466No Hit
GTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAACGTCTCTTACC7330.10674037043131555No Hit
GTATGACTATGTATTCATCATACGATACCTGATAAATCAGGTGGGTTTCCCCATTCGGACATCTGTGAGTCTAAC7320.10659474918925374No Hit
GTTTAGCCCCGTTACATCTTCCGCGCAGACCGACTCGACTAGTGAGCTATTACGCTTTCTTTAAAGGGTGGCTGC7210.1049929155265737No Hit
ACTCACAGATGTCCGAATGGGGAAACCCACCTGATTTATCAGGTATCGTATGATGAATACATAGTCATACGAGGC7210.1049929155265737No Hit
TGTTCAAACAGGATACCACGTGTCCCGTCCTACTCGTTTTCATTGATAAAGCCTTTTCGTGTACGGGGCTATCAC6950.10120676323296632No Hit
CCTTGGCAGTCAGAGGCGATGAAGGACGTGCTAACCTGCGAAAAGCTGTGAGAAGTCGGTAAGAGACGTTAGACT6870.10004179329647174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)