FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002149112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227076
Sequences flagged as poor quality0
Sequence length75
%GC22

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71823.1628177350314433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45492.003294051330832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28141.2392326798076414No Hit
CTTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22010.9692790079092464Illumina PCR Primer Index 12 (96% over 27bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20160.88780848702637No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9940.4377389067977241No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8250.36331448501823177No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7850.3456992372597721No Hit
ATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA6200.27303634025612566Illumina PCR Primer Index 12 (96% over 27bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5470.24088851309693673No Hit
TTATACACATCTCCGAGCCCACGAGACGATACGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4590.20213496802832534RNA PCR Primer, Index 32 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTT3590.15809684863217602No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTT3400.14972960594690765No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2640.11626063520583417No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.10921453610245027No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.10481072416283535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCTTTTTTTTTTTTTTTTTTTTTTTTTT2310.1017280558051049No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)