FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282799
Sequences flagged as poor quality0
Sequence length20-100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70952.5088490411918003No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36461.2892549124996906No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28010.9904561190103219No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28010.9904561190103219No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21650.7655614058041224No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18610.658064561755876No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17900.6329583909419765No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC15210.5378378282808638No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10130.3582049441476101No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG9550.3376956778489316No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9130.32284414018437124No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT8870.3136503311539291No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT7620.2694493261998805No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7580.2680348940413509No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7360.2602555171694384No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.22772357752325859No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6100.21570090417575732No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6050.21393286397759537No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT5720.20226379866972655No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC5180.18316896452957754No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4920.17397515549913542No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4770.16867103490464957No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4740.16761021078575242No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC4700.16619577862722287No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4360.15417310527972164No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA4160.14710094448707386No Hit
ATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.14674733644744148No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4080.14427208017001475No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC3990.14108960781332322No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3850.13613909525846982No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3800.13437105506030783No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3790.13401744702067545No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3780.13366383898104306No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC3770.13331023094141067No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA3720.13154219074324874No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG3710.13118858270361636No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT3690.13048136662435159No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG3670.12977415054508679No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC3610.12765250230729247No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA3610.12765250230729247No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA3570.1262380701487629No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3470.12270198975243901No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3430.12128755759390945No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT3170.11209374856346734No Hit
ATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3060.10820406012751108No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA3000.10608241188971672No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC2990.10572880385008433No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA2960.10466797973118717No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC2940.10396076365192239No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG2890.10219272345376043No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2890.10219272345376043No Hit
ATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.10148550737449566No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2840.1004246832555985Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)