FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346196
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99792.8824712012848215No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT19200.5545991288172019No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT14170.40930571121561193No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG12610.3642445319992143No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA10530.30416295971068413No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG9090.26256802504939397No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG8760.2530358525228483No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTT7800.22530589608198826No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC7710.2227062126656576No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG7270.20999664929693007No Hit
ATGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAATTTAAGCATATC7260.20970779558400443No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG6600.1906434505309131No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT6090.1759119111717062No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGAC5720.16522432379345803No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA5430.1568475661186149No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC5290.1528036141376561No Hit
GACCGGGCGTGGATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGG5250.15164819928595363No Hit
GTCTGATAGCGTGCTGCACGCGAGCTTCGAAAGGGAATCGGGTTAAAATT4980.14384914903696172No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC4980.14384914903696172No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCG4970.1435602953240361No Hit
GACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGAC4940.1426937341852592No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGGGC4820.13922748963015172No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACC4710.1360500987879698No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGG4550.1314284393811598No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGC4270.12334053541924227No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA4220.12189626685461415No Hit
ATCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG4080.11785231487365538No Hit
GTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTTTTCTGTTTAA4050.11698575373487852No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA4010.11583033888317601No Hit
GCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCC3950.11409721660562225No Hit
CAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCG3930.113519509179771No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT3810.11005326462466347No Hit
CTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA3810.11005326462466347No Hit
CTCCTACTCATCGGGGCCTGGCACTTGCCCCGACGGCCGGGTATAGGTCG3720.10745358120833284No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT3710.10716472749540723No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3680.10629816635663034No Hit
TTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACTCTTCCCGAGGC3640.10514275150492784No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCA3600.10398733665322533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)