FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149311

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149311
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences346196
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80612.3284497798934707No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT15290.4416573270632821No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15020.4338582768142901No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA8830.2550578285133277No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG8680.2507250228194433No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG7190.20768581959352506No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6640.19179886538261562No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG6440.1860217911241031No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG6250.1805335705785162No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.17071254433904495No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC5810.16782400720978868No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTT5710.16493547008053241No Hit
ATGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAATTTAAGCATATC5510.15915839582201988No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4970.1435602953240361No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA4740.1369166599267467No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCG4620.13345041537163918No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT4540.13113958566823417No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC4290.12391824284509352No Hit
GACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGAC4280.12362938913216791No Hit
GTCTGATAGCGTGCTGCACGCGAGCTTCGAAAGGGAATCGGGTTAAAATT4210.12160741314168853No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC4120.11900772972535789No Hit
GACCGGGCGTGGATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGG4070.11756346116072976No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGAC4030.11640804630902726No Hit
TCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTGC3950.11409721660562225No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGGGC3800.10976441091173786No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACC3780.1091867034858866No Hit
ATCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG3520.10167650694982033No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG3470.1002322383851922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)