FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002149363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002149363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249335
Sequences flagged as poor quality0
Sequence length20-100
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137765.525096757374617No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33061.3259269657288388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20550.8241923516554034Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19020.7628291254737603No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17990.721519241181543No Hit
ATGGGAAGCAGTGGTATCAACGC15230.6108247939519121No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11480.460424729781218Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.44478312310746587No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10250.4110935087332304No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT8560.34331321314697094Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7780.31202999979946655Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7740.3104257324483125No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC6970.2795435859385967Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6940.2783403854252311No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6600.2647041129404215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6450.25868811037359374Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.23743156797080234No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG5480.2197846271081076No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5350.21457075821685684No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5230.20975795616339465No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5190.20815368881224056No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG5000.20053341889425874Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4580.183688611707141Clontech Universal Primer Mix Long (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4520.1812822106804099Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4390.17606834178915917No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG4210.16884913870896587Clontech Universal Primer Mix Long (96% over 26bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA3930.15761926725088735No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3910.15681713357531032Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3900.15641606673752181No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT3790.1520043315218481No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT3750.15040006417069404No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.14598832895502037No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGA3390.1359616580103074Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGT3230.12954458860569112Clontech Universal Primer Mix Long (100% over 23bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG3160.12673712074117152No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3150.126336053903383No Hit
ATACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.12593498706559447No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA3030.12152325184992079Clontech SMART CDS Primer II A (96% over 26bp)
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3000.12032005133655523No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3000.12032005133655523Clontech Universal Primer Mix Long (96% over 25bp)
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC2990.11991898449876671No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATG2970.1191168508231897Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA2890.11590831612088154Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2870.1151061824453045Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAG2830.11350191509415045No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2700.10828804620289971No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA2700.10828804620289971Clontech Universal Primer Mix Long (95% over 21bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC2690.1078869793651112No Hit
CATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2640.10588164517616862No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2580.1034752441494375Clontech Universal Primer Mix Long (96% over 26bp)
ATACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2570.10307417731164899No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2560.10267311047386046Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA2560.10267311047386046No Hit
ATGGGAAGCAGTGGTATCAAC2550.10227204363607194No Hit
ATACATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2500.10026670944712937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)